单 位：
水产科学重庆市市级重点实验室;西南大学动物科技学院水产系
关键词：
豚鼠气单胞菌(Aeromonas caviae);大鲵(Andrias davidianus);中草药;抑菌作用
摘 要：
应用二倍稀释法测定了诃子、五倍子、石榴皮等16种中草药及其复方对大鲵(Andrias davidianus)致病性豚鼠气单胞菌(Aeromonas caviae)的最小抑菌浓度和最小杀菌浓度,应用棋盘法设计16种中草药组合的120种双联复方和10种中草药组合的120种三联复方,评估了复方的体外抑菌作用。结果表明:16种中草药对大鲵致病性豚鼠气单胞菌均有一定程度的抑菌作用,抑菌作用强弱依次为诃子、五倍子、石榴皮、大黄、槐角、覆盆子、黄芩、金樱子、肉苁蓉、丁香、川牛膝、赤芍、知母、防风、天葵子及罗汉果;在120个中草药双联复方中,5.8%为显著协同作用,82.6%为协同作用,11.6%为相加作用;在120个中草药三联复方中,29.2%为显著协同作用,87.5%为协同作用。因此,合理的中草药配伍能提高对大鲵致病性豚鼠气单胞菌的体外抑菌作用。
译 名：
Inhibitory effects of sixteen kinds of Chinese herbal medicine and their compounds on pathogenic Aeromonas caviae from cultured Andrias davidianus in vitro
作 者：
LING Kong;WANG Han;DING Shi-hua;WU Xing-zhen;Key Laboratory of Aquatic Science of Chongqing/College of Animal Science and Technology,Southwest University;
关键词：
Aeromonas caviae;;Andrias davidianus;;Chinese herbal medicine;;antibacterial activity
摘 要：
To study the inhibitory effects of sixteen kinds of Chinese herbal medicine and their compounds on pathogenic Aeromonas caviae from cultured Andrias davidianus in vitro,the minimum inhibitory concentration( MIC) and the minimum bactericidal concentration( MBC) of single drug and the compounds on pathogenic A. caviae from A. davidianus were measured with agar double dilution method. Then sixteen kinds of chinese herbal medicine were mixed one by one based on chessboard experimental method to get one hundred and twenty kinds of compounds of Chinese herbal medicine. The results showed that sixteen kinds of Chinese herbal medicine had antibacterial effect at certain extent on the pathogenic bacteria. Terminalia chebula Retz and Rhus chinensis Mil had the best antibacterial activity, followed by Pomegranate Rind,Rheum palmatum L, Sophora japonica L, Rubus idaeus, Scutellaria baicalensis, Rosa laevigata, Cistanche deserticola and Syringa Linn,Radix Cyathulae,Paeonia veitchii,Anemarrhena asphodeloides,Divaricate Saposhnikovia Root,Muskroot Semiaquilegia Root and Momordica grosvenori had the worst antibacterial activity. The study indicated that the rational use of Chinese herbal medicine could increase the inhibitory effects of the drug on A. caviae from A. davidianus in vitro drug Antibacterial activity.
