Postharvest Biological Control of Bacillus amyloliquefaciens NCPSJ7 on Apple Ring Rot

HUANG Ling-ling;QIU Ji-ying;TANG lin;CHEN Xiang-yan;LIU Xiao-yong;ZHOU Qing-xin;WANG Yong-hui;CHEN Lei-lei;Institute of Agro-Food Science and Technology,Shandong Academy of Agricultural Sciences;School of Life Science,Shandong Normal University;Hedong Agriculture Bureau of Linyi;

Bacillus amyloliquefaciens NCPSJ7;;apple ring rot;;antibacterial protein;;natamycin;;biological control

In order to estimate the inhibition effects of Bacillus amyloliquefaciens NCPSJ7 on Physollospora piricola and the biological control effects on apple ring rot,bacterial suspension,fermentation broth and antibacterial protein of strain NCPSJ7 selected as test samples,the inhibition effects of the test samples on physollospora piricola LW182 were studied using oxford cup diffusion and inoculation pathogen on punched fruit methods. Moreover,a fruit preservation test was adopted to investigate the effects of the test samples on the biological control of apple ring rot. Results showed that all test samples had varying degrees of inhibition on physollospora piricola LW182,and compared with bacterial suspension and fermentation broth,the antibacterial protein possessed relatively poor inhibition effect on physollospora piricola LW182 which was inoculated on plate,and absolutely better inhibition effect when inoculated on punched apple fruit,mainly manifested as apple rot and browning reduction and mycelial growth suppression of physollospora piricola LW182. Moreover,the antibacterial protein had very good control effect on apple ring rot,which was equivalent with natamycin,while the fermentation broth almost had no effect on apple ring rot. Bacterial suspension,fermentation broth and antibacterial protein of Bacillus amyloliquefaciens NCPSJ7 all had inhibitory effects on physalospora piricola LW182,at the same time,the antibacterial protein of NCPSJ7 had very good practical application value for postharvest biological control of apple ring rot.