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Position: Home > Articles > New Method for Studying Plant Gene Function Acta Agriculturae Boreali-Sinica 2014,29 (1) 109-113

植物基因功能研究新方法

作  者:
彭陈;王瑞;李万昌;郭士伟
单  位:
江苏省农业科学院农业生物技术研究所;河南师范大学生命科学院;江苏省农业生物学重点实验室;江苏省农业科技实业总公司
关键词:
基因功能;TALEN;CRISPR/Cas系统
摘  要:
TALEN(Transcription activator-like effector nuclease)和CRISPR/Cas系统(Clustered regularly interspaced short palindromic repeats,CRISPR.CRISPR-associated,Cas)是近年来发现的基因功能研究的新方法,且已经成功地在动物、植物及微生物等多个领域得到成功应用。它们可以对DNA双链进行切割,从而启动细胞内的DNA修复机制,以此来实现对靶位点的基因修饰。相较于传统方法,它们具有特异性高、操作简便及打靶效率高等特点。主要从结构、功能及作用机制等方面阐述这2种新基因功能的研究方法。
译  名:
New Method for Studying Plant Gene Function
作  者:
PENG Chen;WANG Rui;LI Wan-chang;GUO Shi-wei;Institute of Biotechnology,Jiangsu Academy of Agricultural Sciences,Provincial Key Laboratory of Agrobiology;Jiangsu Agricultural Scientific Industry Co.;College of Life Sciences,Henan Normal University;
关键词:
Gene function;;TALEN;;CRISPR/Cas system
摘  要:
TALEN(Transcription activator-like effector nuclease) CRISPR / Cas system(Clustered regularly interspaced short palindromic repeats,CRISPR.CRISPR-associated,Cas) have recently emerged as new technique for targeted genome modifications.They have been successfully applied in many fields such as animals,plants and microorganism.They can generate a double-strand break(DSB).Repair of these DSBs by intracellular DNA repair mechanisms enables to achieve the target gene modification.Compared with traditional method,TALEN and CRISPR / Cas system were stronger specificity,manipulated more easily and higher targeting efficiency.Here we report the construction,function and mechanism of the TALEN and CRISPR / Cas system.

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