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Position: Home > Articles > Phylogenetic Analysis of the 16S rDNA of a Strain Isolated from Diseased Larva of Anoplophora glabripennis(Motsch.) Journal of Anhui Agricultural Sciences 2008,36 (18) 182-183+192

罹病光肩星天牛幼虫分离菌株的16S rDNA系统发育分析

作  者:
邓彩萍;刘红霞;闫喜中;武旭霞;骆有庆
单  位:
山西农业大学农学院;北京林业大学省部共建森林培育与保护教育部重点实验室;呼和浩特职业学院生物应用工程系
关键词:
光肩星天牛;粘质沙雷氏菌;16S rDNA;系统发育
摘  要:
[目的]研究从罹病光肩星天牛[Anoplophora glabripennis(Motsch.)]幼虫刻槽中分离的菌株BH-1的16S rDNA系统发育及该菌株杀虫特性。[方法]常规细菌鉴定,将BH-1接种健康天牛后观察杀虫效果并通过设计特异引物扩增其16S rDNA序列,进行测序及同源性分析。[结果]用1010cfu/ml BH-1菌液接种2龄天牛幼虫8 d后致死率达72.7%。BH-1与GenBank收录的Serratia marcescens的16S rDNA序列的同源性达99.5%,同时结合细菌的部分常规鉴定结果,鉴定出BH-1菌株为粘质沙雷氏菌(S.marcescens)。[结论]BH-1的发现为天牛的生物防治提供了参考。
译  名:
Phylogenetic Analysis of the 16S rDNA of a Strain Isolated from Diseased Larva of Anoplophora glabripennis(Motsch.)
作  者:
DENG Cai-ping et al(Key Laboratory for Silviculture and Conservation of Education Ministry,Beijing Forestry University,Beijing 100083)
关键词:
Anoplophora glabripennis(Motsch.);Serratia marcescens;16S rDNA;Phylogeny
摘  要:
[Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis(Motsch.) [Method] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis(Motsch.) for observing insecticidal effect,further 16S rDNA was amplified by the specific primers for sequencing and homology analysis.[Result] The mortality of second instar of Anoplophora glabripennis(Motsch.) reached 72.7% 8 d after 1010 cfu/ml BH-1 was inoculated.The homology of 16S rDNA sequences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%.Combined with the results of routine identification,BH-1 was identified as S.marcescens.[Conclusion] BH-1 could be used for biological control of Anoplophora glabripennis(Motsch.).

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