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当前位置: 首页 > 文章 > 烟青虫核型多角体病毒的复制和染病后血淋巴蛋白的变化 昆虫学报 1993,36 (4) 423-429+514
Position: Home > Articles > THE MULTIPLICATION OF HEL1OTHIS ASSULTA NPV AND HOST HEMOLYMPH CHANGES AFTER NPV INFECTION Acta Entomologica Sinica 1993,36 (4) 423-429+514

烟青虫核型多角体病毒的复制和染病后血淋巴蛋白的变化

作  者：
邓塔;蔡秀玉
单  位：
中国科学院动物研究所
关键词：
烟青虫;核型多角体病毒;复制;中肠;气管上皮;血淋巴
摘  要：
用烟青虫(Heliothis assulta)核型多角体病毒感染5龄初烟青虫幼虫,以染病后24、48、72、96、120小时分别测定了虫体血淋巴蛋白浓度的变化。幼虫染病后24小时血淋巴蛋白浓度要高于同期对照组,72小时后染病幼虫血淋巴蛋白浓度较对照急剧下降。在染病及对照血淋巴样品中电泳分析(PAGE)三种蛋白(普通蛋白、糖蛋白和脂蛋白)均可分别染出22条、3条和3条带。分析结果表明,在虫体正常生长代谢过程中发生变化的主要蛋白可能大多为糖脂复合蛋白,但病毒的侵染能抑制这些变化的产生。电镜观察染病毒后幼虫的中肠组织和气管上皮组织,发现中肠染病轻微,不形成多角体。气管上皮细胞感染情况表明其属于对NPV感染较为敏感的组织之一,并在虫体染病后的病毒二次感染上可能起着重要作用。
译  名：
THE MULTIPLICATION OF HEL1OTHIS ASSULTA NPV AND HOST HEMOLYMPH CHANGES AFTER NPV INFECTION
作  者：
DENG TA TSAI XIU-YU(Institute of Zoology, Academia Sinica, Beijing 100080)
关键词：
Heliothii assu/ta-NPV-replication-Mid-gut-tracheal matrix-hemolymph
摘  要：
The pathological changes of Heliothis assulta were examined after infection with nuclear polyhedrosis virus (NPV). The concentration of hemolymph protein in infe-cted larvae was found to be higher than that in the control at 24 hr and decreased sharply 72 hr after infection. For healthy larvae, the concentration of hemolymph protein peaked at 72 hr and 120 hr.The PAGE pattern revealed that there were 22, 3, and 3 bands of general protein, lipoprotein and glycoprotein respectively isolated from hemolymph samples from both infected larvae and the control. There was close relationship among the three types of protein. The concentrations of some of the main proteins from the control became higher at 48 hr and 120 hr while these of corresponding bands from infected larvae remained unchanged, suggesteng that the syntheses of these proteins were inhibited by NPV infection.Various cytological changes of mid-gut cells and tracheal matrix were detected by electron microscopy. No virus was found entered columnar cell from microvilli. The mid-gut cells were infected slightly and at later stage. No polyhedron was formedThe trachael matrix was sensitive to the infection of NPV and subsequently played an important role in repeated infection.

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