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Position: Home > Articles > Bioinformatic Analysis of PEPC in C_3 and C_4 Plants Hubei Agricultural Sciences 2011 (12) 2558-2562

C_3和C_4植物中PEPC的生物信息学分析

作  者：
吴梅;张边江;杨平;王荣富;陈全战
单  位：
南京晓庄学院生物化工与环境工程学院;安徽农业大学生命科学学院
关键词：
C3植物;C4植物;PEPC;生物信息学
摘  要：
PEPC是C4光合途径中的关键酶之一,为研究C3和C4植物PEPC功能上的差异,利用一系列的生物信息学软件分别分析了4种C3和C4植物PEPC基因编码蛋白的氨基酸组成、等电点、结构域、二级结构、空间结构等性质,并进行两类植物蛋白质的比对。结果表明,PEPC基因编码蛋白为不稳定蛋白质;二级结构主要是以无规则卷曲为主。同时揭示出C3和C4植物PEPC存在一定差异。用ESyPred3D的建模服务器进行PEPC结构的三维建模,预测得到一个同源性较高的蛋白质结构数据1JQO chain A,同源性为99.7%,从而构建目标序列的三级结构。利用PROCHECK对建模结果进行检测,模拟得到的玉米PEPC蛋白质的三维结构的氨基酸残基有94.2%位于Ramachandran点图中合理区域,模拟得到的玉米PEPC的三维结构是可靠的。
译  名：
Bioinformatic Analysis of PEPC in C_3 and C_4 Plants
作  者：
WU Mei1,2,ZHANG Bian-jiang1,YANG Ping1,WANG Rong-fu2,CHEN Quan-zhan1(1.College of Biochemistry and Enviromental Engineering,Nanjing Xiaozhuang University,Nanjing 211171,China; 2.College of Life Science,Anhui Agriculture University,Hefei 230036,China)
关键词：
C3 plant;C4 plant;PEPC;bioinformatics
摘  要：
To explore the functional differences between C3 and C4 PEPC,PEPC proteins of four different C3 and C4 plants were analyzed by various bioinformatic tools to predict the protein properties,such as amino acids composition,pI,domains,secondary and spatial structure;and the PEPC protein sequences of C3 and C4 plants were aligned.The results showed that the PEPC proteins were unstable and the secondary structures were mainly composed of random coil,indicating some differences between PEPCs of C3 and C4 plants.A highly homologous(99.7%) protein structure data 1JQO chain A was predicted by three-dimensional structure modeling of PEPC by ESyPred3D,thus facilitated the tertiary structure building of target sequence.The tertiary structure model of Zea mays PEPC was further checked by PROCHECK programmer,and showed that 94.2% of the amino acid residues were located in the most favored regions in Ramachandran plot,indicating that the simulated three-dimensional structure of Zea mays PEPC was reliable.

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