Position: Home > Articles > Residue Dynamics of Chlorsulfuron in Soil Determined byAntibody Immobilized Direct Competitive ELISA
Chinese Journal of Pesticide Science
2000,2
(2)
57-62
固相抗体直接竞争ELISA法测定氯黄隆在土壤中的残留动态
作 者：
刘曙照;袁树忠;徐暄;钱传范
单 位：
农业部农药化学及农药使用技术重点开放实验室;扬州大学植物保护系
关键词：
氯黄隆;土壤;残留;酶联免疫吸附测定(ELISA)
摘 要：
采用包被抗体直接竞争 EL ISA法测定了氯黄隆在盆土中的残留动态和农田土壤中氯黄隆的残留量。结果表明 :EL ISA法测定土壤中氯黄隆的检测限 <0 .1ng/ g,标准添加回收率为 85.4 %～ 118% ,变异系数为 8.95%～ 16 .14% ,符合残留分析的要求。在本实验条件下 ,氯黄隆在土壤中的半衰期为 2 2～ 2 6 d,6 0 d降解 80 %以上。麦茬大田连续两年使用氯黄隆2 2 .5g/ hm2 、4 5g/ hm2 ,第一年麦收时中性沙质土壤和弱碱性沙壤土中氯黄隆的残留量分别为0 .2 4、0 .56 ng/ g和 0 .30、0 .96 ng/ g,第二年分别为 0 .2 8、0 .6 7ng/ g和 0 .38、1.0 9ng/ g,与平行进行的高效液相色谱法、生物测定法测定结果基本一致。
译 名：
Residue Dynamics of Chlorsulfuron in Soil Determined byAntibody Immobilized Direct Competitive ELISA
作 者：
Liu Shuzhao Yuan shuzhong Xu Xuan(Department of Plant Protection, Yangzhou University, Yangzhou 225009)Qian Chuanfan( Key Laboratory of Pesticide Chemistry and Application Technology, Department of Applied Chemistry, China Agricultural University, Beij
关键词：
Chlorsulfuron, Soil, Residue, Enzyme linked immunosorbent assay (ELISA)
摘 要：
A highly specific and sensitive direct competitive (immobilized antibody, enzyme tagged hapten) ELISA was developed to rapidly monitor the residue dynamics of chlorsulfuron in potted soil and cropland soil. The limit of detection was lower than 0.1 ng/g, the recoveries were 85.4%~118%, the CVs(%) were 8.95%~16.14% and the half lives were 22, 26 days for chlorsulfuron in potted soil at 10, 1000 ng/g spiking levels. The residues, at the time of wheat harvest, were 0.24 ng/g and 0.56 ng/g in sandy soil from nonirrigated cropland, 0.30 ng/g and 0.96 ng/g in sandy loam from wheat rice land for the first year, 0.28 ng/g and 0.67 ng/g, 0.38 ng/g and 1.09 ng/g for second year when 22.5g /hm 2 and 45g /hm 2 of chlorsulfuron had been used to control wheat land weed for two years. The results from ELISA were well correspondent with that from HPLC and bioassay.
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