作 者：
韦建兴;梁兆强;欧阳康;白安斌;覃绍敏;钟雅婷;韦丽丽;陆芹章;吴健敏
单 位：
广西大学动物科学技术学院;南宁市水产畜牧兽医局;广西兽医研究所
摘 要：
为了解细环病毒(Torque teno virus,TTV)在广西猪群中的感染情况,本研究运用Nest-PCR方法,对2009—2011年采自广西140个规模猪场的156份血液、流产胎儿及肺脏、脾脏、肾脏、肝脏、淋巴结等组织样品进行检测,并对阳性样品的非编码区(UTR)进行克隆测序及遗传进化分析;同时对部分样品进行猪圆环病毒2型(PCV2)、猪繁殖与呼吸综合征病毒(PRRSV)、典型猪瘟病毒(CSFV)、伪狂犬病病毒(PRV)检测及细菌的分离鉴定。结果发现,广西猪群中TTV总感染率达到93.6%,TTV2的感染率(76.9%)不仅明显高于TTV1(16.7%),且毒株间遗传变异较大。TTV多与PCV2和PRRSV混合感染,且以与PRRSV混合感染率更高(64.29%)。猪群中存在2重、3重,甚至4重TTV与其他病毒的混合感染。临床上TTV与细菌的混合感染(或细菌继发感染)以链球菌和副猪嗜血杆菌多见。本研究证实在广西猪群中存在TTV感染,且存在普遍的TTV与PRRSV、PCV2和CSFV混合感染。
译 名：
Detection of Procine Torque teno Virus,and Investigation and Analysis of Mixed Infection with Many Pathogens
作 者：
WEI Jian-xing;LIANG Zhao-qiang;OU YANG Kang;BAI An-bin;QIN Shao-min;ZHONG Ya-ting;WEI Li-li;LU Qin-zhang;WU Jian-min;College of Animal Sciences and Technology,Guangxi University;Guangxi Veterinary Research Institute;Nanning Bureau of Aquatic Husbandry and Veterinary;
关键词：
Torque teno virus(TTV);;mixed infection;;investigation and analy sis
摘 要：
To investigate the infection of Torque teno virus(TTV)in pigs in Guangxi,a total of 156 samples of blood,aborted fetuses,lungs,spleens,kidneys,livers and lymph nodes from 140 farms were detected by Nest-PCR.Cloning,sequencing and genetic evolution analysis were conducted on the UTR in positive samples,some of which were also detected for porcine circovirus 2(PCV2),porcine reproductive and respiratory syndrome virus(PRRSV),classical swine fever virus(CSFV) and pesudorabies virus(PRV),followed by separation and identification of bacteria.The findings were that the total infection rate of TTV was 93.6% ,in which the infection rate of TTV2 reached 76.9% ,not only being remarkably higher than that of TTV1(16.7% )but also with higher genetic variation among strains.TTV generally mixed with PCV2 and PRRSV, and the rate of mixed infection with PRRSV was higher(64.29% ).There were double,triple or even quadruple TTV mixed infection with other viruses in pigs.Clinically,more Streptococcus pneumoniae and Haemophilus parasuis were detected in mixed infection(or secondary infection)of TTV and bacteria.This study proved that there were TTV infection among swines in Guangxi, and PRRSV,PCV2 and CSFV were more commonly seen in such mixed infections.
