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当前位置: 首页 > 文章 > 水稻osvdac基因RNAi表达载体的构建及遗传转化 安徽农业科学 2011,39 (28) 17176-17178
Position: Home > Articles > Construction and Genetic Transformation of RNAi Expression Vector of osvdac Genes in Rice Journal of Anhui Agricultural Sciences 2011,39 (28) 17176-17178

水稻osvdac基因RNAi表达载体的构建及遗传转化

作  者：
江晨;程钢;刘学群;谭艳平;周杰;王春台
单  位：
中南民族大学生命科学学院生物技术国家民委重点实验室
关键词：
水稻;osvdac基因;RNA干涉;Gateway技术
摘  要：
[目的]构建水稻osvdac3和osvdac5基因RNA干涉表达载体,获得转干涉载体的转基因水稻植株。[方法]采用TRIzol法提取水稻幼苗总RNA,以反转录的cDNA为模板,扩增得到osvdac3和osvdac5的分别靠近5’端和3’端约500～600bp的特异序列,用Gateway重组克隆技术构建RNA干涉表达载体,采用农杆菌介导的愈伤组织侵染法进行遗传转化。[结果]成功构建水稻2个osvdac3和2个os-vdac5基因RNA干涉表达载体,并获得转osvdac3干涉载体的阳性植株。[结论]干涉osvdac3和osvdac5的植株为研究其功能提供材料。
译  名：
Construction and Genetic Transformation of RNAi Expression Vector of osvdac Genes in Rice
作  者：
JIANG Chen et al(Key Lab for Biotechnology of State Ethnic Affairs Commission,College of Life Science,South-Central University for Nationalities,Wuhan,Hubei 430074)
关键词：
Rice(Oryza sativa);osvdac;RNA interference;Gateway recombination cloning technology
摘  要：
[Objective] The research aimed to construct the RNAi expression vectors of osvdac3 and osvdac5 gene,and to obtain transgenic plants with the RNAi vectors.[Method] The total RNA was extracted from rice seedlings with TRIzol and reverse transcripted to cDNA.4 special fragments about 500-600 bp nearby 5' and 3' of osvdac3 and osvdac5 gene were amplified with the cDNA template.The gateway recombination cloning technology was used to construct the RNAi exprefssion vector for 4 fragments.The recombinant RNAi expression vector plasmids were transformed into the callus through agrobacterium-mediated approach.[Result] 4 RNAi expression vectors of the osvdac3 and osvdac5 gene were constructed successfully.And these RNAi expression vectors were introduced into rice variety Yuetai B,and some transgenic plants were obtained.[Conclusion]The transgenic plants with RNAi for osvdac3 and osvdac 5 provided the matercal for studying their function.

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