Position: Home > Articles > Cloning and sequence analysis of the VP gene of the capsid protein of China BQCV-JL1 strain from Black queen cell virus
Heilongjiang Animal Science and Veterinary Medicine
2015
(21)
48-51
黑蜂王台病毒中国BQCV-JL1株衣壳蛋白VP基因克隆及序列分析
作 者：
王向辉;宋战昀;郑言;隋佳辰;杨倩;张健;王振国
单 位：
吉林出入境检验检疫局;吉林农业大学
关键词：
蜜蜂黑蜂王台毒;分离株;VP基因;同源性;遗传进化树
摘 要：
为了研究黑蜂王台病毒在中国的发展情况,在吉林省内疑似蜜蜂黑蜂王台病病料中分离到1株BQCV毒株,记为中国BQCV-JL1(KP119603),对该分离株提取RNA,采用RT-PCR技术扩增其VP基因。结果表明:VP基因测序结果与Gen Bank中报道的BQCV VP基因序列同源性在89%~99%之间。将VP基因序列与Gen Bank中报道的17个序列进行系统遗传进化树分析,其中与日本分离株4(AB605360)、日本分离株3(AB605359)、日本分离株2(AB605358)及日本分离株Ac1(AB638414)、日本分离株Am3(AB638413)、日本分离株Am2(AB638412)株同源性最高(99%),而与南非分离株(AF183905)同源性最低(89%)。
译 名：
Cloning and sequence analysis of the VP gene of the capsid protein of China BQCV-JL1 strain from Black queen cell virus
作 者：
WANG Xiang-hui;SONG Zhan-yun;ZHENG Yan;SUI Jia-chen;YANG Qian;ZHANG Jian;WANG Zhen-guo;Jilin Agricultural University;Jilin Entry-Exit Inspection and Quarantine Bureau;
关键词：
Black queen cell virus in honey bees;;isolate;;VP gene;;homology;;phylogenetic tree
摘 要：
To study the development of the Black queen cell virus( BQCV) in China,a strain of BQCV was isolated from a pathological material with suspected BQCV from Jilin province,and named China BQCV-JL1( KP119603). The isolate was used to extract its RNA,and then a RT-PCR technique was used to amplify its VP gene. The results showed that the sequencing result of the VP gene shared 95% to 99% homology with the sequences of the VP gene of BQCV reported in Gen Bank. A systematic phylogenetic tree was used to analyze the gene sequence of the VP gene with 17 sequences reported in Gen Bank. It was found that China BQCV-JL1 shared highest homology( 99%) with the Japanese strain 4( AB605360),Japanese strain 3( AB605359),Japanese strain 2( AB605358),Japanese strain Ac1( AB638414),Japanese strain Am3( AB638413),Japanese strain Am2( AB638412) and shared lowest homology( 89%) with the South Africa strain( AF183905).
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