Position: Home > Articles > Purification of the hepatoprotective fraction from Hippophae rhamnoides L.subsp.turkestanica
Science and Technology of Food Industry
2014,35
(13)
236-240
中亚沙棘保肝有效成分纯化工艺研究
作 者：
李寅庆;阿布力米提·伊力;阿吉艾克拜尔·艾萨
单 位：
中国科学院大学;新疆特有药用资源利用省部共建国家重点实验室培育基地;中国科学院干旱区植物资源化学重点实验室
关键词：
236;中亚沙棘;大孔吸附树脂;纯化;总三萜;总酚
摘 要：
目的:建立中亚沙棘保肝有效部位纯化方法。方法:以总三萜和总酚吸附量和解吸量为指标,通过静态吸附与解吸实验筛选适宜大孔吸附树脂;并通过动态吸附和解吸实验考察最优工艺条件。结果:适宜纯化工艺条件为:AB-8大孔吸附树脂,上样浓度为0.067g生药/mL,上样液pH为6,上样液流速为2BV/h,上样量为9~10BV,洗脱剂为70%乙醇,洗脱剂用量为3.5BV。纯化后总三萜含量49.3%,总酚含量36.1%,总有效部位纯度达到85.4%。
译 名：
Purification of the hepatoprotective fraction from Hippophae rhamnoides L.subsp.turkestanica
作 者：
LI Yin-qing;YILI Abulimit;AISA Haji-akber;Key Laboratory of Plant Resources and Chemistry of Arid Zone,State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences;University of Chinese Academy of Sciences;
关键词：
Hippophae rhamnoides L.subsp.Turkestanica;;macroporous resin;;purification;;total triterpenoids;;total phenolics
摘 要：
Objective: To establish the purification craft of the hepatoprotective fraction from Hippophae rhamnoides L.subsp.turkestanica. Method: The adsorption and desoption ability of the total triterpenoids and phenolics were carried out to select the appropriate marcoporous resin.Then,the dynamic adsorption and desoption experiments were used to study the appropriate purification craft.Result: AB-8 resin was better than other resins.The optimum purification conditions: sample solution concentration 0.067g /mL,9~10BV sample solution in pH6,flow rate 2BV/h,Elution solvent 70%,ethanol washing 3.5BV.Under these optimum conditions,the purified product contained total triterpenoids 49.3% and total phenolic 36.1%,the total effective fraction content was 85.4% 。
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