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当前位置: 首页 > 文章 > 马铃薯品种和组织对基因组测序深度分布模式的影响 甘肃农业大学学报 2016,51 (3) 43-48
Position: Home > Articles > Effects of potato cultivars and tuber sections on the sequencing depth pattern in genome Journal of Gansu Agricultural University 2016,51 (3) 43-48

马铃薯品种和组织对基因组测序深度分布模式的影响

作  者：
张国栋;刘柏林;李修宝;司怀军;李修庆
单  位：
加拿大农业部马铃薯研究中心;日照市农业科学院;甘肃省作物遗传改良与种质创新重点实验室
关键词：
马铃薯;DNA测序;小序列片段;参考基因组;覆盖程度;品种差别;组织特异性
摘  要：
【目的】探讨品种和组织对马铃薯基因组测序深度分布的影响.【方法】对‘布尔班克’和‘云薯107’2个品种的薯块芽端和茎端进行了深测序,建立了在参照基因组上的测序深度分布图.【结果】发现这些分布在品种间有明显差别(例如在三号染色体中部和尾部),在同一品种的DNA样本间很类似,但芽端和茎端在二号染色体有明显差别.这些结果说明测序深度的分布主要受品种基因组本身所影响,也受薯块上部位影响,而且在很大程度上可以重复.【结论】这些结果能帮助设计试验和合适利用测序深度.
译  名：
Effects of potato cultivars and tuber sections on the sequencing depth pattern in genome
作  者：
ZHANG Guo-dong;LIU Bai-lin;LI Xiu-bao;SI Huai-jun;LI Xiu-qing;Gansu Provincial Key Laboratory of Arid-land Crop Science,Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement,Gansu Agricultural University;College of Life Science and Technology,Gansu Agricultural University;Potato Research Centre,Agriculture and Agri-Food Canada;Rizhao Academy of Agricultural Sciences;
关键词：
potato;;second generation DNA sequencing;;small sequence fragments;;reference genome;;coverage depth;;cultivars difference;;tissue-specific variation
摘  要：
【Objective】The paper aims to explore the effects of potato cultivars and tuber section on the Illumina sequencing depth pattern in genome.【Method】Deep sequencing was performed on tuber bud and stem end of two potato cultivars including‘Russet Burbank'and ‘Yunshu 107',establishing distribution map based on sequencing in genome.【Result】The sequencing depth distribution differed significantly between the two cultivars such as in the middle and end of reference chromosome 3,was highly similar among DNA samples within the same cultivars,but clearly different in reference chromosome 2.These results indicated that sequencing depth distribution was mainly impacted by genotypes,also by tuber position and could be repeated to a large extent.【Conclusion】These findings may help to the design and data interpreta-tion in biological research using the second generation sequencing approach.

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