Position: Home > Articles > Transgenic Ingredients Detection of GM Rapeseed from Import Grains
Seed
2006,25
(4)
18-20
谷物中掺杂油菜籽的转基因成分检测
作 者：
张舒亚;潘良文
单 位：
上海出入境检验检疫局
关键词：
进境谷物;杂质;转基因油菜籽;PCR检测
摘 要：
杂质是谷物及其加工产品中转基因成分污染的一个重要来源,本文对来源于进境谷物中的50份油菜籽样品进行了转基因成分(gox基因、pa t基因和bar基因)检测。通过常规定性PCR检测,在31份样品中检出转基因油菜籽,检出率为62%。本研究使用AB I 7700定量PCR仪对油菜籽样品DNA进行了实时荧光PCR定性检测分析,在32份样品中检出含有转基因油菜籽,检出率为64%。在今后要加强进出口谷物中杂质作物的转基因成分检测。
译 名：
Transgenic Ingredients Detection of GM Rapeseed from Import Grains
作 者：
Zhang Shuya,Pan Liangwen(Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai,200135)
关键词：
Imported grains Foreign materials GM rapeseed PCR detection
摘 要：
The presence of transgenes in 50 rapeseed samples from imported grains were determined by conventional PCR and real-time PCR.The conventional qualitative PCR results showed that GMO derived material existed in 31 samples,and the percentage is 62%.Furthermore,real-time qualitative PCR was conducted by ABI 7700 Sequence Detection System,and GMO derived material was found in 32 samples,and the percentage is 64%.it is necessary to detect transgenes in foreign materials in the future.
导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
