作 者：
米丽班·霍加艾合买提;毛居代·亚尔买买提;热孜耶·喀日;热合满·艾拉
单 位：
新疆师范大学化学化工学院科研处;新疆师范大学生命科学学院;新疆农业大学食品科学与药学学院
摘 要：
以凝血酶比活力为响应值,在单因素实验的基础上,筛选出激活时间、激活温度、Ca2+浓度三个对激活影响较大的因素,响应面法进行优化,并验证优化方案。结果表明,凝血酶原激活工艺的优化参数为:激活时间为2.8h,温度为32℃,Ca+2浓度为0.11mol/L。在此条件下,凝血酶比活力为51.16U/mg。
译 名：
Optimization of activation condition of prothrombin from horse blood by using response surface methodology
作 者：
Mihriban·Hojahmat;Mawjuda·Yarmanmat;Reziye·Kari;Rehman·Ala;College of Chemistry and Chemical Engineering,Scientific Research Department,Xinjiang Normal University;Department of Life,College of Life Science,Xinjiang Normal University;College of Food Sciences,Xinjiang Agricultural University;
关键词：
prothrombin;;activation;;response surface methodology
摘 要：
Based on single factor experiment,three main factors were screened out,including activation time,activation temperature,and concentration of Ca2 +. Optimization of these factors by using response surface methodology,the results showed that the optimum activation conditions of prothrombin were as follows:activation time 2.8h,temperature 32℃,concentration of Ca+20.11mol/L. Under these conditions,the specific activity of thrombin was 51.16U/mg.
