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当前位置: 首页 > 文章 > PrP106-126对星形胶质细胞层黏连蛋白和纤黏连蛋白表达的影响 动物医学进展 2012,33 (12) 38-42
Position: Home > Articles > Effects of PrP 106-126 on Expression of Laminin and Fibronectin in Astrocyte Progress in Veterinary Medicine 2012,33 (12) 38-42

PrP106-126对星形胶质细胞层黏连蛋白和纤黏连蛋白表达的影响

作  者：
李玉荣;武现军;吴浩;陈立功;霍书英;高玉红
单  位：
保定市畜牧水产局;河北农业大学动物医学院
关键词：
PrP106-126;星形胶质细胞;小胶质细胞BV-2;层黏连蛋白;纤黏连蛋白
摘  要：
星形胶质细胞增生是朊病毒病早期的主要病理学特征。PrP106-126(prion protein peptide106-126)具神经毒性,导致星形胶质细胞增生。层黏连蛋白(laminin,LN)和纤黏连蛋白(fibronectin,FN)是星形胶质细胞胞外基质的主要成分。利用PrP106-126和PrP106-126制备的小胶质条件培养基(conditioned medium from microglia,MiCM)分别作用于体外培养的星形胶质细胞,应用RT-PCR和Westernblot技术探讨PrP106-126对星形胶质细胞的增殖及其胞内LN、FN表达的影响。试验分为5个处理(空白对照、Scr对照、PrP106-126、MiCM、MiCMPrP106-126)。结果表明,PrP106-126可促进星形胶质细胞增殖、LN和FN的表达,但促增殖和FN表达作用有赖于活化小胶质细胞细胞因子的共同参与。
译  名：
Effects of PrP 106-126 on Expression of Laminin and Fibronectin in Astrocyte
作  者：
LI Yu-rong1,2,WU Xian-jun1,WU Hao3,CHEN Li-gong1,HUO Shu-ying1,GAO Yu-hong1(1.College of Veterinary Medicine,Hebei Agricultural University,Baoding,Hebei,071001,China; 2.Hebei Engineering and Technology Research Center of Veterinary Biological Products,Baoding,Hebei,071001,China; 3.Animal Husbandry and Fishery Bureau of Baoding,Baoding,Hebei,071051,China)
关键词：
prion peptide 106-126;astrocyte;BV-2;laminin(LN);fibronectin(FN)
摘  要：
Astrogliosis is a hallmark of prion disease,but the metabolic alterations of astrocytes remain poorly documented.A synthetic peptide corresponding to amino acid 106-126 of prion protein(PrP) has been shown to be toxic to neurons.In this study,VB-2 cells and astrocytes were used,the effects of PrP 106-126 on astrocytes were investigated in vitro.The experiment was divided into five groups(Control,Scr,PrP106-126,MICM,MiCMPrP106-126).The proliferation of astrocytes was significantly(P<0.01) increased when grown in media conditioned with PrP 106-126 from microglia.The expression of laminin(LN) and fibronectin(FN) was examined at both mRNA and protein levels at 48 h.The results showed that exposure of astrocytes to PrP 106-126 enhanced the cell viability and the expression of LN and FN.The increase of FN in astrocyte cultures required cytokines previously released by activated microglia.

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