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枳椇叶片再生体系的优化研究

作  者:
孙叶芳;郑琪;孙骏威;陈珍;周丹
单  位:
绍兴市农业科学研究院;台州学院生命科学学院;中国计量学院生命科学学院生物系
关键词:
枳椇;植物生长调节剂;再生苗叶片;不定芽诱导;生根诱导
摘  要:
以枳椇组培再生苗的叶片为外植体,进行离体培养植株再生研究。结果表明:外植体宜选择叶色浓绿的成熟叶片,诱导愈伤组织和不定芽的最佳培养基为1/2MS+BA 2.0mg/L+IAA 1.0mg/L,诱导率可超过85%,增殖率可超过6.0;最佳生根培养基为1/4MS+NAA 0.1mg/L,添加2%蔗糖,生根率可达95%以上。
译  名:
Study on Optimum for Regeneration System of Leaves in Hovenia acerba
作  者:
SUN Ye-fang 1,ZHENG Qi 1,SUN Jun-wei 2,CHEN Zhen 3,ZHOU Dan 2(1.Shaoxing Academy of Agriculture Sciences,Shaoxing,Zhejiang 312003;2.Department of Life Sciences,China Jiliang University,Hangzhou,Zhejiang 310018;3.School of Life Sciences,Taizhou University,Taizhou,Zhejiang 318000)
关键词:
Hovenia acerba;plant growth regulator;leaf disc of regeneration plants;adventitious buds induction;rooting induction
摘  要:
A regeneration protocol for Hovenia acerba were set up via organogenesis from callus derived from leaf disc of regeneration Hovenia acerba plants in vitro.The results showed that the best explants were mature leaves with dark green color,and the best placement way of explants was face-up;the most appropriate medium for the induction of callus and adventitious buds was 1 / 2MS+BA 2.0 mg / L+IAA 1.0 mg / L,with the induction rate more than 85% and proliferation rate more than 6.0;the most appropriate medium for the rooting was 1 / 4MS+NAA 0.1mg / L,with addition of 2%sucrose,in which the rooting rate exceeded 95%.

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