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PCR检测炭疽杆菌的研究

作  者:
肖履中;赵青
单  位:
青海大学农牧学院;青海出入境检验检疫局
关键词:
炭疽杆菌;质粒;PCR;多重PCR
摘  要:
以无毒炭疽芽孢苗 (sterne菌株 )、II号炭疽芽孢苗 ( pasteurII菌株 )、A16R菌苗 (sterne菌株 )为试验菌 ,建立了检测炭疽芽孢杆菌质粒DNA的PCR方法。该方法特异性强 ,敏感性可达1131个菌/ml(无毒炭疽芽孢菌菌株 )和1720个菌/ml(II号炭疽芽孢菌菌株 )。同时建立了可区分炭疽杆菌强、弱毒的多重PCR方法
译  名:
Studies of PCR for Detecion of Bacillus Anthracis
作  者:
Xiao LüZhong1) Zhao Qing2)(1 Faculty of Agriculture and Animal Sciences, Qinghai University, Xining, 810003)(2 Qinghai Entry-Exit Inspection and Quarantine Bureau of the People,s Republic of China,Xining,810000)
关键词:
Bacillus anthracis;Plasmid: PCR; Multiplicate PCR
摘  要:
A PCR for detection of Bacillus anthracis plasmids DNA was established by avirulent Bacillus anthracis .This assay was of high specificity and sensitivity. It could detect 1131 /ml( sterne strain )or 1720 /ml ( Pasteur Ⅱstrain).And the multiplicate PCR assay was established to distinguish virulent and avirulent strain of the Bacillus anthrax.

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