Position: Home > Articles > Content Determination of Rutin and Sophoricoside in FRUCTUS SOPHORAE at Different Harvesting Time
Journal of Anhui Agricultural Sciences
2011,39
(35)
21631-21633
不同时间采摘的槐角中芦丁和槐角苷的含量测定
作 者：
Yuanjun Liu;Wang Zhi-ling;Lingyan Gou;Huimin Liu;Jing Wan
单 位：
Bethune Military Medical College,The Forth Military Medical University,Shijiazhuang,Hebei 050081,China;Bethune Military Medical College,The Forth Military Medical University,Shijiazhuang,Hebei 050081,Chin
关键词：
sophoricoside;rutin;fructus sophorae;mid;harvesting time;the conten
摘 要：
[目的]研究槐角中芦丁和槐角苷含量与生长时间(采摘时间)的关系,确定不同应用目的槐角药材的最佳采摘时间。[方法]在2006～2008年的89、1、0月中旬分别采摘槐角样品,采用无水甲醇连续提取法提取槐角中的总黄酮,并用HPLC法测定提取物中芦丁和槐角苷含量。色谱条件:色谱柱为Diamonsil C18(150 mm×4.6 mm,5μm);流动相为无水甲醇-浓度1%冰醋酸-乙腈(30.4∶64.6∶5,V/V/V);流速为1.0 ml/min;检测波长为260 nm。[结果]8月中旬采摘的槐角中芦丁的含量最高,为(0.87±0.16)%8,月中旬以后采摘的槐角中芦丁的含量逐渐降低;槐角苷在9月中旬到10月中旬达到高峰期,含量分别为(5.76±0.22)%和(5.33±0.39)%,明显高于冬季含量。[结论]槐角中芦丁和槐角苷含量具有明显的季节变化规律,而冬季恰是槐角中芦丁和槐角苷含量最低的时期。根据槐角的不同用途选择合适的采摘时间,为科学利用现有槐角资源提供一定的理论依据。
译 名：
Content Determination of Rutin and Sophoricoside in FRUCTUS SOPHORAE at Different Harvesting Time
作 者：
LIU Yuan-jun et al(Bethune Military Medical College,the Forth Military Medical University,Shijiazhuang,Hebei 050081)
关键词：
FRUCTUS SOPHORAE;Rutin;Sophoricoside;HPLC
摘 要：
[Objective] To investigate the relationship between the rutin,sophoricoside content in FRUCTUS SOPHORAE(originated from the dried fruits of S.japonica) and its growth time(harvesting time) in order to define the optimal harvesting time of FRUCTUS SOPHORAE for different applying purpose.[Method] The fruits of S.japonica were collected once a month from the mid-August to the next mid-January for three consecutive years between 2006 and 2008.The content of rutin and sophoricoside in the extracts obtained from FRUCTUS SOPHORAE by absolute methanol continuous extraction method was simultaneously determined by RP-HPLC.The separation of extracts was carried out on a Diamonsil C18 column(150 mm×4.6 mm,5 μm) with the detection wavelength at 260 nm.Following the injection of extracts,an elution with methanol-1% glacial acetic acid-acetonitrile(30.4∶64.6∶5,V/V/V) as mobile phase was initiated at a flow rate of 1 ml/min.[Result] Rutin peaked in the mid-August,with the level of(0.87±0.16) %,then declined gradually.Sophoricoside,the most predominant components of flavonoids,reached the highest between the mid-September and mid-October,which was(5.76±0.22)% and(5.33±0.39)%,respectively.The content of sophoricoside in FRUCTUS SOPHORAE harvested in autumn was much higher than that in the fruits collected in winter(P<0.05).[Conclusion] The content of rutin and sophoricoside in FRUCTUS SOPHORAE at different development stages had the tendency of seasonal variation,which was the lowest in that harvested in winter.Being known the seasonal variation of the content of rutin and sophoricoside,the optimal harvesting time of S.japonica fruits for different applying purpose could be defined,aiming at providing certain theoretical basis for the scientific utilization of current resources.
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