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当前位置: 首页 > 文章 > 浙江大盘山华顶杜鹃菌根真菌的分离和鉴定 浙江林业科技 2019 (1) 1-8
Position: Home > Articles > Isolation and Identification of Mycorrhizal fungi in Rhododendron huadingense Root in Zhejiang Dapanshan National Nature Reserve Journal of Zhejiang Forestry Science and Technology 2019 (1) 1-8

浙江大盘山华顶杜鹃菌根真菌的分离和鉴定

作  者：
刘亚;王盼;周钰鸿;陈江芳;陈子林;唐光大
单  位：
磐安县林业局;浙江省大盘山国家级自然保护区管理局;浙江省磐安县风景旅游管理局;华南农业大学林学与风景园林学院
关键词：
华顶杜鹃;杜鹃花类菌根;菌根真菌分离;浙江大盘山
摘  要：
于2016年5月在浙江省大盘山国家级自然保护区的3处采样地,每处采集5株华顶杜鹃Rhododendron huadingense的1～2支侧根,培养分离华顶杜鹃的菌根真菌,然后结合菌落形态特征和r DNAITS序列分析进行鉴定。结果表明:华顶杜鹃根系内共分离到165个菌株,分为22个菌落形态类型;结合r DNA ITS序列分析,最终分为20个不同的菌种类型,其中19个菌种类型属于子囊菌门Ascomycota,包括8个属于粪壳菌纲Sordariomycetes和6个属于锤舌菌纲Leotiomycetes;3个优势菌种分别为Phialocephala fortinii,Ilyonectria radicicola和某粪壳菌纲真菌的近缘种,分别占菌株总数的41.82%,22.42%和10.91%;其余17个菌种类型合计只占总数的24.85%。
译  名：
Isolation and Identification of Mycorrhizal fungi in Rhododendron huadingense Root in Zhejiang Dapanshan National Nature Reserve
作  者：
LIU Ya;WANG Pan;ZHOU Yu-hong;CHEN Jiang-fang;CHEN Zi-Lin;TANG Guang-da;Pan'an Administration of Tourism of Zhejiang;Administration of Dapanshan National Natural Reserve of Zhejiang;Pan'an Forestry Bureau of Zhejiang;College of Forestry and Landscape Architecture, South China Agricultural University;
关键词：
Rhododendron huadingense;;ericoid mycorrhiza(ERM);;Mycorrhizal fungi isolation;;Dapanshan National Nature Reserve
摘  要：
Three sample plots were established in Dapanshan National Nature Reserve, Zhejiang province in May of 2016. 5 Rhododendron huadingense were selected for 1-2 lateral roots each. Ericoid mycorrhiza(ERM) was cultured from collected roots and studied by morphological characteristics and molecular identification of r DNA ITS sequence. The result showed that 165 mycorrhizal fungal strains were isolated which were classified into 22 types based on colony characteristics. r DNA ITS demonstrated that these mycorrhizal fungi could be identified into 20 species,among them, 19 species belonging to Ascomycota, including 8 Sordariomycetes, 6 Leotiomycetes. The dominant 3 species were Phialocephala fortinii, Ilyonectria radicicola and Sordariomycetes sp., respectively accounting for 41.82 %, 22.42% and 10.91% of the total strains. The lest 17 species occupied only 24.9%.

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