Cytological Traits of F_2 Hybrids of Chinese Spring-Ae.cylindryca Host. Gametocidal Chromosome 2C Disomic Addition×Wheat-A. cristatum (L.) Beauv. Addition

WANG Jing-chang1,2, LIU Wei-hua2, CHENG Xue-jiao2, GUO Yong2, SU Jun-ji1,2, YANG Xin-ming2, GAO Ai-nong2, CHAI Shou-cheng1, LI Li-hui2(1College of Agronomy, Northwest Agricultural and Forestry University, Yangling 712100, Shaanxi; 2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081)

Wheat-Agropyron addition lines; Gametocidal chromosome; Cytological traits

【Objective】In order to validify the chromosome variations induced by gametocidal chromosome while it was transferred into wheat-A. cristatum (L.) Beauv. addition lines, to construct wheat-A. cristatum (L.) Beauv.translocation lines, the F2 PMCs chromosome variations of five crosses of wheat-A. cristatum (L.) Beauv. addition lines hybridized with Chinese spring-Ae.cylindryca Host. gametocidal chromosome 2C disomic addition lines were observed. 【Method】The chromosome variations of F2 PMCs of young ears were observed by using the routine method while P chromatin was detected by GISH.【Result】 The results showed that plurivalents, multi-univalents,lagging chromosomes and chromosomes fragments were observed frequently in PMCI metaphase in all of F2 hybrid crosses, chromosome bridges in most of crosses and ring chromosomes in very few crosses.The mean abnormal chromosome frequencies of F2 PMCs were as follows: 36.5% cells with multiunivalent, 15.8% cells with plurivalent, 31.6% cells and 11.7% cells with fragments and chromosome bridges, respectively. Frequent microkernels, multifid quadrants, and retrogression were observed in quadrants phase in certain crosses. Self seed setting percentage of F2 was 31.47% in mean. There were 2.7% plants could be induced to produce translocations by gametocidal chromosome. The P genome usually wasunivalent in some F2 crosses detected by GISH.【Conclusion】The gametocidal chromosome 2C had a typical feature in inducing chromosome fragments at a high rate and killing some gametal cells. The gametocidal chromosome 2C was efficient in inducing chromosome variations when it was transferred into wheat-A. cristatum (L.) Beauv. addition lines. Different inducing effects and rates were observed when transferred into different wheat-Agropyron addition lines.