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当前位置: 首页 > 文章 > 棉铃虫成虫感觉神经元膜蛋白(SNMP)表达及其与Gqα的关系(英文) 昆虫学报 2010,53 (8) 36-41
Position: Home > Articles > Expression pattern of sensory neuron membrane protein(SNMP) in adults of Helicoverpa armigera(Lepidoptera:Noctuidae) and its relation with Gqα Acta Entomologica Sinica 2010,53 (8) 36-41

棉铃虫成虫感觉神经元膜蛋白(SNMP)表达及其与Gqα的关系(英文)

作  者：
苏宏华;王桂荣;郭予元
单  位：
植物病虫害生物学国家重点实验室;中国农业科学院植物保护研究所
关键词：
棉铃虫;昆虫嗅觉;信号转导;SNMP;Gqα;酵母双杂交技术;空间表达
摘  要：
昆虫嗅觉对昆虫的取食、求偶、寻找产卵场所、搜寻寄主或猎物至关重要。有研究认为感觉神经元膜蛋白(SNMP)和Gq蛋白α亚基(Gqα)参与信号转导。为了阐明snmp的空间表达情况并确定Gqα是否与SNMP直接结合,我们采用半定量RT-PCR技术对snmp在棉铃虫Helicoverpa armigera(Hbner)成虫的触角、头、胸、腹、足、翅、喙、下颚须和下唇须的分布情况进行了研究,并利用酵母双杂交技术对SNMP与Gqα的关系进行了研究。半定量RT-PCR研究发现,snmp不仅在棉铃虫成虫的触角中表达,而且在喙、下颚须、下唇须及足上都有表达。利用酵母双杂交技术研究发现,Gqα与SNMP不直接相互作用,Gqα不是SNMP的直接下游结合蛋白。这些研究结果说明,SNMP不仅参与气味识别而且也参与味觉识别;SNMP可能与气味受体(OR)形成复合物,然后与Gqα结合,这需要我们进一步深入的研究进行证明。
译  名：
Expression pattern of sensory neuron membrane protein(SNMP) in adults of Helicoverpa armigera(Lepidoptera:Noctuidae) and its relation with Gqα
作  者：
SU Hong-Hua,WANG Gui-Rong,GUO Yu-Yuan(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
关键词：
Helicoverpa armigera; insect olfaction; signal transduction; SNMP; Gqα; yeast two-hybrid technique; spatial expression
摘  要：
Insect olfaction is important for insects to detect the food,reproductive partners,oviposition sites,hosts,prey or predators. Sensory neuron membrane protein (SNMP) and Gq alpha subunit (Gqα) were thought to be involved in signal transduction in insect olfaction. In order to make clear the spatial expression pattern of snmp and understand whether Gqα is the direct downstream binding protein of SNMP,semi-quantitative RT-PCR method was used to learn the expression pattern of snmp in antennae,head,thorax,abdomen,legs,wings,proboscis,labial palpus and maxillary palpus of adult Helicoverpa armigera,and yeast two-hybrid technique was used to investigate the relationship between SNMP and Gqα. Semi-quantitative RT-PCR results showed that snmp was expressed not only in the antennae of adult H. armigera,but also in proboscis,labial palpus,maxillary palpus and legs. Yeast two-hybrid screening results showed that Gqα did not directly interact with SNMP. These results suggest that SNMP plays some roles not only in odor detection but also in taste recognition,and SNMP maybe forms a complex with an olfactory receptor (OR) and then binds with Gqα,which needs our further investigation.

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