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Position: Home > Articles > Tissue Culture and Rapid Propagation of Yunnan Wild Rhodiola crenulata Journal of Anhui Agricultural Sciences 2009,37 (17) 67+69

云南野生大花红景天的组织培养与快速繁殖

作  者:
王云美;王元忠;张仲凯
单  位:
云南省农业科学院;云南省农业科学院生物技术与种质资源研究所
关键词:
大花红景天;组织培养;快速繁殖
摘  要:
[目的]为掌握云南野生大花红景天的组织培养与快速繁殖技术。[方法]将叶片接种于培养基MS+BA 2 mg/L+IAA 0.2 mg/L,花芽接种在培养基MS+KT 2 mg/L上,茎尖接种在培养基MS+BA 2 mg/L+IAA 1 mg/L上,幼茎接种在培养基MS+KT 2 mg/L+IAA 2mg/L上,在培养条件下进行培养。[结果]以叶片为外植体诱导芽效果最好,分化率达100%;幼茎、茎尖与花芽分化时间长,且分化率较低,不宜作为快繁技术的外植体;在培养基MS+NAA 0.2 mg/L上,生根率达100%;通过炼苗后,成活率达70%左右。[结论]为大花红景天的快速繁殖提供了理论依据。
译  名:
Tissue Culture and Rapid Propagation of Yunnan Wild Rhodiola crenulata
作  者:
WANG Yun-mei et al (Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650231)
关键词:
Rhodiola crenulata;Tissue culture;Rapid propagation
摘  要:
[Objective] The study aimed to research tissue culture and rapid propagation technique of Yunnan wild Rhodiola crenulata.[Method] Inoculating leaves with culture medium MS+BA 2 mg/L+IAA 0.2 mg/L,inoculating blossom bud with culture medium MS+KT 2 mg/L,inoculating stem point with culture medium MS+BA 2 mg/L+IAA 1 mg/L,inoculating immature stem with culture medium MS+KT 2 mg/L+IAA 2 mg/L,cultivated them under the condition.[Result] The effect was best using leaves as explants induce stem,induction percentages reached 100%;the induction of immature stem,stem point,blossom bud not only took more time,and the percentages also too low,they were not suit to take as explants for rapid propagation;the shoot induction percentage of culture medium MS+NAA 0.2 mg/L reached 100%;after having been hardened the regeneration plates could survive at 70%.[Conclusion] Providing basis for rapid Propagation of Rhodiola crenulata.

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