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当前位置: 首页 > 文章 > 3种粒线虫多重PCR检测方法 植物病理学报 2006,36 (6) 508-511
Position: Home > Articles > Multiple-PCR detection on three species of Anguina genus Acta Phytopathologica Sinica 2006,36 (6) 508-511

3种粒线虫多重PCR检测方法

作  者：
马以桂;王金成;谢辉;周春娜;杜宇;黄国明;李芳荣
单  位：
华南农业大学;云南出入境检验检疫局;深圳出入境检验检疫局;天津出入境检验检疫局
关键词：
剪股颖粒线虫;小麦粒线虫;维氏粒线虫;DNA;多重PCR
摘  要：
剪股颖粒线虫[Anguina ag rostis(S teinbuch,1799)F ilipjev,1936]、小麦粒线虫[Anguina tritici(S teinbuch,1799)F ilipjev,1936]和维氏粒线虫[Anguina w evelli(Van den Berg,1985)S idd iq i,2000]都是重要的植物病原线虫,它们在成熟种子和虫瘿中的虫态通常是幼虫,而这3种线虫的幼虫形态非常相似,难以根据其特征对它们进行快速准确的种类鉴定。本研究根据这3种线虫的rDNA-ITS区域序列,分别设计筛选了特异性引物AgrF1/AgrR1、TriF1/TriR1、W evF1/W evR1,构建了这3种线虫单条幼虫多重PCR检测体系,获得3个大小差异明显的片段,表明这些引物设计合理,适合这3种线虫的快速准确检测。
译  名：
Multiple-PCR detection on three species of Anguina genus
作  者：
MA Yi-gui~(1,2),WANG(Jin-cheng~2),XIE Hui~1,ZHOU Chen-na~1,DU Yu~3,HUANG Guo-ming~2,LI Fang-rong~4(~1South China Agricultural University,Guangzhou 510642,China;~2Tianjin EntryExit Inspection and Quarantine Bureau,Tianjin 300456,China;~3Yunnan EntryExit Inspection and Quarantine Bureau,Kunming 650228,China;~4Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen 518010,China)
关键词：
Anguina agrostis;Anguina tritici;Anguina wevelli;DNA;multiple-PCR
摘  要：
Anguina agrostis,Anguina tritici and Anguina wevelli are important pests of agriculture.However,only juveniles are generally found in seed galls and similar in morphology.Three pairs of primers,named as AgrF1/AgrR1,TriF1/TriR1 and WevF1/WevR1,were designed based on rDNA-ITS nucleotide sequences of A.agrostis,A.tritici and A.wevelli.PCR techniques were developed and improved for detection of the three species with DNA extrated from single juvenile as templates.The amplified products for A.agrostis,A.tritici and A.wevelli were 499,617 and 377 bp in size and showed a strong unique band in electrophoresis respectively.This study indicated that the multiple-PCR technology could satisfy the need of detection of A.agrostis,A.tritici and A.wevelli.

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