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Position: Home > Articles > STUDY ON COMPARISON OF THREE RAPID DIAGOSIC METHODS FOR RABBIT HEMORRHAGIC DISEASE Chinese Journal of Animal and Veterinary Sciences 1992,23 (4) 361-365

兔出血症三种快速诊断法比较研究

作  者:
刘永德;汪毅;徐俊;施蔡培;陈智龙
单  位:
上海农学院动物科学系
关键词:
兔出血症;免疫酶染色法;斑点酶联免疫吸附试验;间接荧光染色法
摘  要:
本试验对快速诊断兔出血症(RHD)的免疫酶染色法(IES),斑点酶联免疫吸附试验(Dot-ELISA)与间接荧光染色法(IFS)进行了比较。试验表明,三种快速诊断法对17份实验感染兔肝材料的检测结果与血凝试验(HA)的阳性符合率分别为88.2%、88.2%及93.3%,阴性符合率均为100%;对4份脏器材料检测结果证明,兔出血症病毒(RHDV)含量依次为;肝>脾>肾>肺>心肌。肝触片及快诊膜分别在4℃和室温放置1个月后,其检测结果不受影响。三种快速诊断对RHDV的检测具有很高的特异性、敏感性和稳定性。Dot-ELISA具有经济、简便、快速、敏感、准确的优点。
译  名:
STUDY ON COMPARISON OF THREE RAPID DIAGOSIC METHODS FOR RABBIT HEMORRHAGIC DISEASE
作  者:
Liu Yongde, Wang Yi, Xu Jun, Shi Caipei, Chen Zhilong ( Department of Animal Science, Shanghai Agricultural College )
关键词:
RHD, IES, Dot-ELISA, IFS
摘  要:
Immunoenzyme Stainning ( IES ),Dot-Enzyme Linked Immunosorbe-nt Assay ( Dot-ELISA ) and Indirect Fluorescent Stainning ( IFS ) for the rapid diagnosis of Rabbit Hemorrhagic Disease ( RHD ) were compared. Livers samples of 17 rabbits infected experimently with Rabbit Hemorrhagic Disease Virus ( RHDV)were tested by the three methods, and compared with Hemagglutination ( HA ) test. The result showed that the positive coincidence rates were 88.2%,88.2%, and 93.3% , respectively, and the negative ones were all 100%.The three methods were proved to have the advantages of high specificity, sensitivity and stability.

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