作 者：
李科友;唐德瑞;李林;朱海兰;赵忠;侯琳
摘 要：
以西黄松成熟胚为外植体诱导不定芽,在GD+9.85～19.70μmol.L-16-BA+0.0～14.42μmol.L-1NAA上不定芽诱导率最高达65.8%,平均增殖率为7,最大增殖率达10;不定芽形成有2种途径,即子叶直接形成不定芽和子叶组织再分化形成不定芽;NAA不利于外植体不定芽的诱导;不定芽的生长和扩繁采用不加生长调节剂的1/2GD和1/2SH培养基;培养基中加入适量的活性炭有利于不定芽和根的生长。不定嫩梢在1/2GD和1/2SH附加不同浓度NAA和GA3的培养基上进行生根诱导,试验结果表明:NAA对不定根的形成起主要作用,在1/2GD+28.84μmol.L-1NAA+4.17μmol.L-1GA3培养基中不定梢的生根率为16.7%。在离体培养条件下,以西黄松成熟胚为外植体获得了再生植株。
译 名：
Adventitious Bud Induction and Plantlet Regeneration in vitro from Mature Zygotic Embryos of Ponderosa Pine
作 者：
Li Keyou1 Tang Derui1 Li Lin 1,2 Zhu Hailan1 Zhao Zhong1 Hou Lin1(1.College of Forestry,Northwest A & F University Yangling 712100;2.Applied Science and Technology College,Guilin University of Electronic Technology Guilin 541004)
关键词：
Ponderosa Pine(Pinus ponderosa);mature zygotic embryos;in vitro;plantlet regeneration
摘 要：
Adventitious buds were induced from the excised mature zygotic embryos of Ponderosa Pine(Pinus ponderosa).The highest induction rate(65.8%) of adventitious buds was obtained with 10-fold multiplication at the highest and 7-fold multiplication at an average when mature zygotic embryos were cultured on GD + 9.85～19.70 μmol·L-1 6-BA+ 0.0～14.42 μmol·L-1 NAA.The adventitious buds were initiated or formed directly from the cotyledons.Additional NAA inhibited the formation of adventitious buds.Buds proliferation and elongation were achieved on 1/2GD and 1/2SH without growth regulators.A proper amount of activated charcoal promoted adventitious buds elongation and root growth.The adventitious shoots of Ponderosa Pine were used to induce adventitious roots on 1/2GD and 1/2SH media supplemented with different concentrations of NAA and GA_3.The results showed that NAA played a determinative role in initiating the adventitious roots.Sixteen point seven percent(16.7%) of adventitious shoots was rooted on 1/2GD with 28.84 μmol·L-1 NAA+4.17 μmol·L-1 GA_3.In the present paper,the experimental results showed that roots were successfully induced from cultured mature zygotic embryos of Ponderosa Pine.
