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Position: Home > Articles > Study on the Conditions of Ramie Degumming by Mixed Bacteria Method Hubei Agricultural Sciences 2015 (17) 4253-4256

苎麻混菌脱胶工艺研究

作  者:
陈其国;鞠守勇;尹喆;李莉
单  位:
武汉职业技术学院生物工程学院
关键词:
苎麻[Boehmeria nivea(L.)Gaudich.];混菌;脱胶
摘  要:
将培养36 h后的B3、B5种子液和培养48 h后的M1种子液,按照顺序间隔12 h接种10 m L于装有3 g苎麻[Boehmeria nivea(L.)Gaudich.]原样、100 m L原始脱胶液的250 m L三角瓶中,通过单因素和正交试验,得到苎麻最佳混菌脱胶工艺为按照B3B5M1接种顺序,原始脱胶液pH 6,培养温度35℃,转速为150 r/min,脱胶时间60 h,在此条件下,苎麻脱胶率最高可以达到29.9%。
译  名:
Study on the Conditions of Ramie Degumming by Mixed Bacteria Method
作  者:
CHEN Qi-guo;JU Shou-yong;YIN Zhe;LI Li;Bioengineering College, Wuhan Institute of Technology;
关键词:
ramie[Boehmeria nivea(L.) Gaudich.];;mixed bacteria;;degumming
摘  要:
Three kinds of seed liquid(B3, B5 after cultured for 36 h respectively, and M1 after cultured for 48 h) were inoculated 10 m L into 100 m L of original degumming liquid containing 3 g of ramie in order at the interval of 12 h, and single factor and orthogonal experiment were carried out to determine the best degumming process. The results showed that the optimal conditions for ramie degumming were pH 6,temperature 35 ℃,rotational speed 150 r / min and degumming time 60 h,according to the B3B5M1 inoculation order. Under the above conditions, the highest degumming rate reached to 29.9%.

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