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当前位置: 首页 > 文章 > 寄主凤凰柚对柑橘衰退病病毒甜橙分离株构成的影响 园艺学报 2007,34 (5) 1098-1102
Position: Home > Articles > Influence of Fenghuang Pummelo on the Composition of Citrus tristeza virus Isolates Collected from Sweet Orange Acta Horticulturae Sinica 2007,34 (5) 1098-1102

寄主凤凰柚对柑橘衰退病病毒甜橙分离株构成的影响

作  者：
王志刚;周常勇;周彦
单  位：
中国农业科学院柑橘研究所;西南大学植物保护学院
关键词：
柑橘;柑橘衰退病毒;株系构成;寄主
摘  要：
为研究不同柑橘类型对柑橘衰退病病毒构成的影响,将9个保存在新会橙上的引起甜橙茎陷点症状的柑橘衰退病毒强毒分离株嫁接接种到凤凰柚后,比较了接种前后p25基因的Hinf I RFLP和SSCP变化情况。分离株CT90与CT20接种前后的RFLP组群没有发生变化;RFLP组群发生变化的分离株中有6个由混合组群变为单一组群。此外,除分离株CT90接种前后的SSCP谱带保持不变外,其余8个分离株从新会橙接种到凤凰柚后,SSCP谱带数均呈减少趋势。试验结果倾向于表明某些能在甜橙上增殖的CTV株系在凤凰柚上的增殖受到了抑制。
译  名：
Influence of Fenghuang Pummelo on the Composition of Citrus tristeza virus Isolates Collected from Sweet Orange
作  者：
WANG Zhi-gang~1,ZHOU Chang-yong~(1.2),and ZHOU Yan~2 (1 College of Plant Protection, Southwest University, Chongqing 400716,China;2 Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, China)
关键词：
Citrus;;Citrus tristeza virus;;Composition of isolate;;Host
摘  要：
For the preliminary study on influence of different citrus cultivars on the composition of Citrus tristeza virus(CTV)isolates, the HinflRFLP and SSCP of p25 gene of 9 CTV isolates collected from sweet orange shown stem-pitting symptom were comparatively analysed as those isolates were graft inculated from Xinhui sweet orange to Fenghuang pummelo.The results of RFLP revealed that the Hinf I RFLP group of the isolates CT90 and CT20 were kept the same as in Xinhui sweet orange and in Fenghuang pummelo; RFLP groups of other 6 isolates shown mixed groups in Xinhui sweet orange and single group in Fenghuang pummelo. DNA bands of p25/SSCP of the isolate CT90 kept the same as in Xinhui sweet orange and in Fenghuang pummlo but DNA bands of other 8 isolates maintained in Fenghuang pummlo were less than that of which maintained in Xinhui sweet orange.It seems that the replication of some composition of above 8 CTV isolates collected from sweet orange were inhibited in Fenghuang pummelo.

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