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当前位置: 首页 > 文章 > 奶牛舍环境中气载微生物含量的检测 西北农林科技大学学报(自然科学版) 2010,38 (5) 56-60
Position: Home > Articles > Concentration detection of airborne microbes in cow environments Journal of Northwest A & F University(Natural Science Edition) 2010,38 (5) 56-60

奶牛舍环境中气载微生物含量的检测

作  者：
刘敬博;柴同杰;苗增民;周玉法;刘东燕
单  位：
山东农业大学动物科技学院;山东省泰安市岱岳区畜牧局;泰安市岱岳区畜牧局
关键词：
奶牛舍;气载微生物;培养计数法;DAPI染色法
摘  要：
【目的】准确地定量奶牛舍环境中气载微生物(细菌和真菌)的含量,并评估其健康风险。【方法】利用国际标准的AGI-30空气采样器,以无菌生理盐水为采样介质,收集奶牛舍空气样品,分别用培养计数法和4,6-联脒-2-苯基吲哚(DAPI)染色法,对奶牛舍环境中气载微生物含量进行计数,比较两种方法的差异,并对气载微生物潜在危害进行评估。【结果】培养计数法测得奶牛舍内气载微生物含量为1.14×105~8.32×106CFU/m3,而DAPI染色法为9.3×105~5.93×107CFU/m3,DAPI染色法所测气载微生物含量约是培养计数法的7~733倍。以染色法测得的奶牛舍内气载微生物含量计算,人与奶牛每min吸入的微生物量分别为1.58×105CFU和3.29×106CFU。【结论】染色计数法更能客观地反映环境中的气载微生物含量,有助于对环境的健康风险进行科学评估。
译  名：
Concentration detection of airborne microbes in cow environments
作  者：
LIU Jing-bo1,CHAI Tong-jie1,MIAO Zeng-min1,ZHOU Yu-fa2,LIU Dong-yan2,LI Ming-yong3(1 Collage of Animal Science,Shandong Agricultural University,Tai'an,Shandong 271018,China;2 Farming Bureau of Daiyue District in Taian City,Tai'an,Shandong 271000,China;3 Qingdao Kangda-Eurolap Rabbit Selecition Limited Company,Qingdao,Shandong 266400,China)
关键词：
cow house;airborne microbe;culture counting method;DAPI-staining method
摘  要：
【Objective】 The research was conducted to quantify the concentrations of airborne microbes(bacteria and fungi) in cow houses and assess health risks.【Method】 Air samples of cow environments were collected by international AGI-30 using sterile saline water as sampling media;the concentrations of airborne microbes were counted by culture counting method and 4,6-diamidino-2-phenylindole(DAPI)-staining counting method,respectively.The differences between the two methods were compared.【Result】 According to counting grown colonies,the concentrations of cow houses were within a range of 1.14×105-8.32×106 CFU/m3.The direct counting concentrations were between 9.3×105-5.93×107 CFU/m3.The results of DAPI-staining counting method were about 7-733 times higher than those of culture counting method.Based on the concentration of DAPI-staining counting method,the airborne microbes inhaled by a person and a cow were 1.58×105 CFU/min and 3.29×106 CFU/min,respectively.【Conclusion】 DAPI-staining counting method could reflect the accurate concentrations of airborne microbes in the environment,and assess health risks scientifically.

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