Position: Home > Articles > Determination of Glucose by the Novel Glucose Oxidase Electrode Based on Prussian Blue in Juice Beverages
FOOD SCIENCE
2005,26
(10)
187-190
基于普鲁士蓝的葡萄糖氧化酶电极用于果汁饮料中葡萄糖测定
作 者：
李彤;姚子华;丁良
单 位：
河北大学化学与环境科学学院;河北职工医学院;河北科技大学环境科学与工程学院
关键词：
葡萄糖测定;酶电极;普鲁士蓝;果汁
摘 要：
利用PB修饰电极结合二氧化硅溶胶凝胶技术制备的葡萄糖氧化酶电极应用于果汁饮品中葡萄糖含量的测定研究。以牛血清白蛋白代替葡萄糖氧化酶制得的电极作对照实验,来研究样品基质对测定的影响,另外考察了可能的干扰物对酶电极的干扰情况,实验结果表明,样品基质等对测定没有明显的干扰。测定结果重现性较好,回收率在102.1%～104.2%。
译 名：
Determination of Glucose by the Novel Glucose Oxidase Electrode Based on Prussian Blue in Juice Beverages
作 者：
LI Tong1,YAO Zi-hua2,DING Liang3 (1.College of Environment Science and Engineering , Hebei University of Science and Technology, Shijiazhuang 050018,China;2.College of Chemistry and Environmental Science, Hebei University, Baoding 071002,China;3.Hebei Medical College for Continuing Education, Baoding 071000, China)
关键词：
determination of glucose;enzyme electrode;prussian blue;juice beverage
摘 要：
The novel glucose oxidase electrode based on Prussian Blue(PB)-modified electrode combined with SiO2 sol-gel immobilization has been used to determinate the glucose content in juices and beverages. In this experiment, the BSA electrode made similarly by taking BSA instead of GOD, to be served as the reference electrode, has been employed to study the interference of matrix and interfering species in real sample. The testing results showed that the matrix and interfering species did not produce any observable interference for the assay. The reproducibility was satisfactory and the recovery was found to be in the range of 102.1%～104.2%.
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