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当前位置: 首页 > 文章 > 水稻第6染色体短臂上株高QTL qPH6-1的精细定位 中国水稻科学 2009,23 (5) 470-474
Position: Home > Articles > Fine Mapping of QTL qPH6-1 for Plant Height on the Short Arm of Rice Chromosome 6 Chinese Journal of Rice Science 2009,23 (5) 470-474

水稻第6染色体短臂上株高QTL qPH6-1的精细定位

作  者：
鲍钱江;樊叶杨;於卫东;陈忱;范方军;杜景红;庄杰云
单  位：
杭州师范大学;中国水稻研究所
关键词：
株高;数量性状基因座;精细定位;剩余杂合体;水稻
摘  要：
应用衍生于水稻剩余杂合体的分离群体,开展株高QTL的检测和精细定位。应用1个在水稻第6染色体短臂约7.3 Mb区间分离、背景基本纯合的F2:3群体,种植于海南、浙江两地,检测到2个控制株高的QTL;然后,针对两地间作用稳定的qPH6-1,筛选出3个杂合区间缩小且呈阶梯状排列的单株,衍生F2群体,进一步验证了qPH6-1的作用,并将其界定于距离为96.4 kb的SSR标记RM3414和RM19417之间;最后,应用分离区间进一步缩小且呈阶梯状排列的3个F2群体,将qPH6-1定位于距离为51.7 kb的STS标记Si2925和SSR标记RM19417之间。基因组位置比较结果显示,该基因与所有已定位或克隆的水稻矮秆、半矮秆基因均非等位。
译  名：
Fine Mapping of QTL qPH6-1 for Plant Height on the Short Arm of Rice Chromosome 6
作  者：
BAO Qian-jiang1,2,FAN Ye-yang1,YU Wei-dong2,CHEN Chen1,FAN Fang-jun1,DU Jing-hong1,ZHUANG Jie-yun1,(1 Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China;2 College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310036,China;)
关键词：
plant height;quantitative trait locus;fine mapping;residual heterozygous line;rice
摘  要：
Populations derived from residual heterozygous lines(RHLs) were used for the detection and fine mapping of quantitative trait loci(QTLs) for plant height in rice.An F2:3 population which segregates in a 7.3-Mb region on the short arm of rice chromosome 6 in an isogenic background were transplanted in two trial sites in Hainan and Zhejiang Provinces,respectively.Two QTLs for plant height were detected,of which qPH6-1 exhibited a consistent effect across the two sites.Three plants having smaller heterozygous segments which overlap the qPH6-1 region were selected.Using the three F2 populations produced,qPH6-1 was validated and delimitated to a 96.4-kb region flanked by SSR markers RM3414 and RM19417.Three more F2 populations which segregate in smaller regions covering qPH6-1 were developed.The qPH6-1 locus was delimited to a 51.7-kb region flanked by STS marker Si2925 and SSR marker RM19417.It was also shown that this gene is non-allelic to all the major genes for dwarfism mapped or cloned in rice.

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