中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 高山杜鹃的组织培养快速繁殖技术研究 上海农业学报 2004,20 (3) 15-18
Position: Home > Articles > STUDY ON RAPID PROPAGATION OF Rhododendron lapponicum Acta Agriculturae Shanghai 2004,20 (3) 15-18

高山杜鹃的组织培养快速繁殖技术研究

作  者：
汤桂钧;张建安;蒋建平;胡海峰;何正其
单  位：
上海市闵行区农业科学研究所
关键词：
高山杜鹃;组织培养;快速繁殖
摘  要：
以德国产高山杜鹃的茎段和茎尖进行外植体诱导试验 ,结果显示 ,茎外植体诱导无菌芽效果较好 ,诱导率达 5 0 .0 %。分别比较了不同培养基对外植体诱导、丛生芽增殖及试管苗生根的效果 ,结果表明 ,外植体诱导的适宜培养基是 1/ 4MS +MS铁盐 +维生素B50 .2mg/L +KT1.0mg/L +水解乳蛋白 (CH) 30 0mg/L +蔗糖30 g/L ;丛生芽增殖的适宜培养基是 1 2MS +KT0 .5mg L +水解乳蛋白 (CH) 30 0mg/L +蔗糖 30 g/L ;生根培养基则以 1 2MS +NAA5mg/L +蔗糖 2 0 g/L +活性炭 0 .5 %为宜。试管苗经假植炼苗培育成穴盘苗再移栽定植 ,可确保种苗质量 ,经穴盘培育的高山杜鹃试管苗 ,上盆移栽存活率达 10 0 %。
译  名：
STUDY ON RAPID PROPAGATION OF Rhododendron lapponicum
作  者：
Tang Guijun Zhang Jian'an Jiang Jianping Hu Haifeng He Zhengqi (Agricutural Science Research Institute of Minhang District,Shanghai 201109)
关键词：
Rhododendron lapponicum; Tissue culture; Rapid propagation
摘  要：
The stem apexes and shoots of Rhododendron lapponicum from Germany were used as explants, and the experiment of inducing the plant regeneration was carried out.The results showed that stem shoots had a better effect than that of the stem apexes on inducing the plant regeneration and the inductivity reached 50%.The comparison of the effects of different media on the explant induction,the proliferation of clump sprouts and the test-tube plantlet rooting showed that the best medium for explant induction was 1/4MS+ferric salt (MS)+0.2mg·L -1vitamin B 5+1.0mg·L -1KT+300mg·L -1CH+30g·L -1sucrose,the best for proliferating clump sprouts was 1/2MS+0.5mg·L -1KT+300mg·L -1CH+30g·L -1sucrose,and the best medium for the plantlet rooting was 1/2MS+5mg·L -1NAA+20g·L -1sucrose+activated charcoal 0.5%. The test-tube plantlets were temporarily planted by tray method and then set out, which could improve the quality of the plantlets and make their survial rate reach nearly 100%.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46