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Position: Home > Articles > The Genetic Diversity of the Rhizobia Isolated from Caragana arborescens & C.sinica of Yingkou in Liaoning in China Journal of Shenyang Agricultural University 2007,38 (1) 44-48

辽宁营口地区锦鸡儿与树锦鸡儿根瘤菌遗传多样性研究

作  者:
严雪瑞;陈文峰;陈文新;傅俊范;周如军;隋新华
单  位:
中国农业大学生物学院;沈阳农业大学植物保护学院
关键词:
锦鸡儿;树锦鸡儿;根瘤菌;遗传多样性;16SrDNA PCR RFLP;BOX-PCR
摘  要:
对锦鸡儿与树锦鸡儿根瘤菌进行研究,以了解其系统发育地位及遗传多样性。通过分离、纯化、回接结瘤测定共获得了31株待测菌,并采用16S rDNA PCR RFLP及BOX-PCR等方法对这些菌进行了研究。结果表明,31株待测菌的系统发育地位全部源于中间根瘤菌属(Mesorhizobium spp.),并产生5种不同的rDNA图谱组合。BOX-PCR研究中,待测菌株呈现出较高的多样性,在80%相似性水平上,将待测菌归于17个不同的类型当中。
译  名:
The Genetic Diversity of the Rhizobia Isolated from Caragana arborescens & C.sinica of Yingkou in Liaoning in China
作  者:
YAN Xue-rui1,CHEN Wen-feng2,CHEN Wen-xin2,FU Jun-fan1,ZHOU Ru-jun1,SUI Xin-hua2(1.College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China;2.Department of Biology,China Agricultural University,Beijing 100094,China)
关键词:
Caragana arborescens(Amm.) Lam;Caragana sinica(Buc'hoz) Rehd;rhizobia;genetic diversity;16S rDNA PCR RFLP;BOX-PCR
摘  要:
The rhizobia from Caragana sinica and C.arborescens were studied here for their phylogenic positions and genetic diversity.31 isolates were gotten after separating,purifying and nodulation checking and researched by methods of 16S rDNA PCR RFLP and BOX-PCR.The results indicated that they were belonged to the Mesorhizobium genera with 5 different rDNA types.In addition,they covered 17 patterns of BOX PCR fingerprinting,which implied there were high genetic diversity among these isolates.

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