Identification and Karyotype Analysis of Offspring of F_1 Hybrids to Two Kinds of Clover

REN Shang-jia;WANG Ming-jiu;HUANG Fan;HE Li-jun;BAI Chun-li;College of Ecology and Environmental Science,Inner Mongolia Agricultural University;Grassland Research Institute,Chinese Academy of Agricultural Science;Agricultural College,Inner Mongolia Agricultural University;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences;

Trifolium ambiguum Bieb.cv.Mengnong No.1;;T.repens L.;;F1 hybrids;;root tip chromosome;;karyotype analysis

Hybrids F1from distant hybridization of Trifolium ambiguum Bieb cv. Mengnong No.1 and T. repens were highly sterile plants,which were just flowering instead of seeding over the past two years of the field planting. However, it started to fructification from the third year. In order to explore the seeding mechanism of F1,the technology of root tip chromosome tableting was used to identify the somatic chromosome of F1′s seeds,meanwhile the condition of the tableting technology was explored and optimized. The results turned out that the somatic chromosome number in the seeds of F1ranged from 40 to 48,and some cells of individuals emerged into chromosome doubling phenomenon and mixoploid. The chromosome number of somatic cells in F1hybrids was 40,and 48 in T. ambiguum Bieb cv. Mengnong No.1. The somatic chromosome number of the 6-3′s seeds was 44,which was initially identified as the resulting of backcross of F1hybrids with T. ambiguum Bieb cv. Mengnong No.1,and its karyotype formula was 2n=30m+14sm,which belonged to the type of 2A.