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当前位置: 首页 > 文章 > 山楂海棠嫩茎直接再生芽苗体系及种质离体保存 林业科学研究 2009,22 (6) 140-144
Position: Home > Articles > Preliminary Study on Shoots Regeneration from Tender Stems of Malus komarovii (Sarg.) Rehd. and Germplasm Reservation in vitro Forest Research 2009,22 (6) 140-144

山楂海棠嫩茎直接再生芽苗体系及种质离体保存

作  者：
顾地周;高捍东;张庆增;王玉芳;冯颖
单  位：
农业部特种经济动植物及产品质量监督检验测试中心;通化师范学院生物系;南京林业大学森林资源与环境学院
关键词：
山楂海棠;植株再生;种质离体保存;均匀设计
摘  要：
The tender stems of Malus komarovii were used as explant and the suitable medium compositions were screened through uniform design experiments.The results showed that tissue culture of M.komarovii required different kinds of culture medium in different phases.SH+TDZ 2.45 mg·L-1+NAA 0.06 mg·L-1 for shoots regeneration immediately at base of tender stem,the rate of induction was 98.8%;1/4 SH+KT 0.35 mg·L-1+NAA 0.05 mg·L-1 for rooting,the rate of rooting was more than 97%;1/8 SH+ABA 2.05 mg·L-1+KT 0.50 mg·L-1 for germplasm preservation in vitro,the rate of dormancy was 95.5%.These plant materials could be maintained for 39 months by the methods of promoting dormancy and low nutrients at normal temperature.
译  名：
Preliminary Study on Shoots Regeneration from Tender Stems of Malus komarovii (Sarg.) Rehd. and Germplasm Reservation in vitro
作  者：
GU Di-zhou1,GAO Han-dong2,ZHANG Qing-zeng1,WANG Yu-fang3,FENG Ying1(1.Department of Biology,Tonghua Normal University,Tonghua 134002,Jilin,China;2.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;3.Supervision and Testing Center for Special Economic Animal and Plant Product Quality,Ministry of Agriculture,Jilin 132109,Jilin,China)
关键词：
Malus komarovii;plant regeneration;germplasm reservation in vitro;uniform design
摘  要：
The tender stems of Malus komarovii were used as explant and the suitable medium compositions were screened through uniform design experiments.The results showed that tissue culture of M.komarovii required different kinds of culture medium in different phases.SH+TDZ 2.45 mg·L-1+NAA 0.06 mg·L-1 for shoots regeneration immediately at base of tender stem,the rate of induction was 98.8%;1/4 SH+KT 0.35 mg·L-1+NAA 0.05 mg·L-1 for rooting,the rate of rooting was more than 97%;1/8 SH+ABA 2.05 mg·L-1+KT 0.50 mg·L-1 for germplasm preservation in vitro,the rate of dormancy was 95.5%.These plant materials could be maintained for 39 months by the methods of promoting dormancy and low nutrients at normal temperature.

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