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当前位置: 首页 > 文章 > 利用高通量测序分析侵染贵州火龙果的病毒 植物病理学报 2017,47 (3) 364-369
Position: Home > Articles > High-throughput sequencing analysis of viruses in pitaya from Guizhou Province,China Acta Phytopathologica Sinica 2017,47 (3) 364-369

利用高通量测序分析侵染贵州火龙果的病毒

作  者：
郑乾明;王壮;彭志军;马玉华;蔡永强
单  位：
贵州省果树科学研究所
关键词：
仙人掌科;基因组;核苷酸;序列一致性
摘  要：
对疑似感染病毒的火龙果(Hylocereus undatus Britt.)进行高通量测序,分析病毒种类和相对含量,为火龙果病毒诊断和检测提供理论基础。测序reads经拼接和注释,共3 969条contig注释为病毒,包含44 354 147条reads。10条contig注释为CVX-NTU(JF937699),其含有的reads占所有病毒reads数量的85.18%;10条contig注释为ZyVX(AY366208),占所有病毒reads数量的4.97%;6条contig注释为SchVX(AY366207),占所有病毒reads数量的3.75%;6条contig注释为CVX(AF308158),占所有病毒reads数量的0.26%。拼接获得的CVX-NTU序列完全覆盖CVX-NTU(JF937699)基因组,核苷酸序列一致性为97%。编码的5个ORF核苷酸序列与CVX-NTU(JF937699)一致性为97%~98%,氨基酸序列一致性为97%~99%。拼接获得的CVX-NTU序列与CVX、ZyVX和SchVX的核苷酸序列一致性分别为78%、75%和70%。这是首次利用高通量测序检测火龙果病毒的报道。
译  名：
High-throughput sequencing analysis of viruses in pitaya from Guizhou Province,China
作  者：
ZHENG Qian-ming;WANG Zhuang;PENG Zhi-jun;MA Yu-hua;CAI Yong-qiang;Gui Zhou Institute of Pomology Science;
关键词：
cactaceae;;genome;;nucleotide;;sequence identity
摘  要：
A high-throughput sequencing for pitaya(Hylocereus undatus Britt.) tissues infected potentially by virus was performed,and the type and relative content of viruses were studied,which provided a theoretical basis for virus diagnosis and detection of pitaya.On the whole,3 969 contigs,annotated as virus-related sequence,were produced from the generated 44 354 147 reads by library sequencing and assembly.Among them,10,10,6 and 6 contigs were annotated as CVX-NTU(JF937699),ZyVX(AY366208),SchVX(AY366207) and CVX(AF308158)-related sequences,respectively.Of all virus-related reads,the count percentage of the CVX-NTU,ZyVX,SchVX and CVX-related reads was 85.18%,4.97%,3.75% and 0.26%,respectively.The re-assembled genome sequence of CVX-NTU entirely covered the previously reported CVX-NTU(JF937699) genome.Sequence identity between the two genomes was 97%.The nucleotide sequence identity of 5 ORFs located in the genome between the re-assembled CVX-NTU and the reported CVX-NTU(JF937699) reached to 97%-98%,and the amino acid identity reached to 97%-99%.The sequence identity of the re-assembled CVX-NTU genome with CVX,ZyVX and SchVX were 78%,75% and 70%,respectively.This is the first report of virus detection in pitaya using high-throughput sequencing.

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