Analysis on the Dehydrogenase Activity in Cold-resistant Bacteria in the Processor of Low-temperature Wastewater

JIANG Ming et al (Department of Biology,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157012)

Low-temperature biofilm;TTC-dehydrogenase;Cold-resistant bacteria;Microbial activity

[Objective] The aim was to supply technical support for solving the problem that the sewage was difficult to dispose in north China in winter.[Method] The influences of pH value,temperature and time on the dehydrogenase activity in 8 strains of cold-resistant bacteria isolated,purified and identified from low-temperature biofilm were analyzed by TTC method.[Result] The optimum pH value of the strains S1,S4,S5,S7 and S8 at both optimum temperature and 4 ℃ was 7.5 and that of the other 3 strains at the 2 temperatures were different,but they were in the range of 7.5-8.5.The optimum reaction temperature of the strains S1,S3,S4,S5 and S6 was 30 ℃ and that of the strains S2,S7 and S8 was 20 ℃.Except for the strain S3,the optimum reaction time of the other strains at 4 ℃ was longer than their optimum reaction time at optimum reaction temperature.At 4 ℃,the optimum pH values of the strains S3,S4 and S6 were 8.5,7.5 and 8.0 resp.,their optimum reaction time were 0.6,12.0 and 0.5 h resp.and their highest dehydrogenase activities were 7.19,6.73 and 7.70 mg(TF)/g(SS) resp.[Conclusion] The dehydrogenase activities of the strains S3,S4 and S6 were higher and they were suitable to be used in processing low-temperature sewage.