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当前位置: 首页 > 文章 > 洋葱成花素基因AcFT的克隆与表达分析 园艺学报 2014,41 (5) 907-914
Position: Home > Articles > Molecular Cloning and Expression Analysis of AcFT Florigen Gene in Allium cepa Acta Horticulturae Sinica 2014,41 (5) 907-914

洋葱成花素基因AcFT的克隆与表达分析

作  者：
刘彬昕;王勇;陈典;韦贺远
单  位：
农业部东北地区园艺作物生物学与种质创制重点实验室;东北农业大学园艺学院
关键词：
洋葱;AcFT基因;RACEPCR;荧光定量;表达分析
摘  要：
以春化后但尚未抽薹的洋葱品系‘1007’的叶片为试材,采用RT-PCR结合RACE的方法克隆得到了洋葱成花素基因的cDNA全长序列,将其命名为AcFT(GenBank登录号为KF913629)。该基因cDNA全长791 bp,开放读码框为534 bp,推测其编码的蛋白由177个氨基酸残基组成,等电点为7.89,分子量为19.8 kD。将AcFT与大葱、鸢尾等植物的成花素氨基酸序列进行同源性比对,结果显示洋葱与其他植物的氨基酸序列同源性均在65%以上,其中洋葱与大葱的成花素氨基酸的同源性高达98.31%。实时荧光定量RT-PCR分析结果表明,洋葱AcFT基因在抽薹前后的根、叶鞘、叶片、假茎和花序等不同部位均有表达,尤以春化后抽薹前的叶片中表达量最高。
译  名：
Molecular Cloning and Expression Analysis of AcFT Florigen Gene in Allium cepa
作  者：
LIU Bin-xin;WANG Yong;CHEN Dian;WEI He-yuan;Department of Horticulture,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Northeast Region,Ministry of Agriculture,The Northeast Agriculture University;
关键词：
Allium cepa;;AcFT gene;;RACEPCR;;fluorescence quantitative;;expression analysis
摘  要：
The florigen gene which designated as AcFT isolated from the leaf of Allium cepa line,‘1007'from vernalization period to bolting period(GenBank accession No.KF913629).The full-length cDNA of florigen gene in A.cepa was obtained by RT-PCR and Rapid Amplification of cDNA Ends.The cDNA sequence of AcFT was 791 bp long and the open reading frame was 534 bp long which could encode a putative protein of 177 amino acids with a molecular weight of 19.8 kD and a theoretical pI of 7.89.Compared and analyzed the amino acid sequence of florigen gene with Allium fistulosum,Iris fulva and so on,the A.cepa shared over 65% nucleotide sequence similarity with others and the similarity of amino acids with the florigen gene in A.fistulosum is 98.31%.Fluorescence quantitative RT-PCR system followed was established to examine the expression of AcFT.As a result,the AcFT gene from A.cepa detected during th e different tissues that was root leaf sheath,leaf,stem and inflorescence from bolting to vernalization,and it was highly expressed in leaves before bolting after vernalization.

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