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当前位置: 首页 > 文章 > 甘蓝型油菜LOX2基因在MeJA、BTH和核盘菌诱导下的表达 河南农业科学 2010,2010 (7) 22-25
Position: Home > Articles > Brassica napus LOX2 Gene Expression Induced by Methyl Jasmonate(MeJA),Benzothiadiazole(BTH),and Sclerotinia sclerotiorum Journal of Henan Agricultural Sciences 2010,2010 (7) 22-25

甘蓝型油菜LOX2基因在MeJA、BTH和核盘菌诱导下的表达

作  者：
任秋红;黄军艳;毛晗;田保明;刘胜毅
单  位：
郑州大学;中国农业科学院
关键词：
油菜;菌核病;MeJA;BTH;LOX2
摘  要：
利用荧光定量PCR技术,检测了甘蓝型油菜脂氧合酶基因LOX2在化学激素(MeJA和BTH)处理和核盘菌(Sclertinia sclerotiorum)接种后的表达差异,以及核盘菌诱导处理后LOX2基因在不同油菜菌核病抗性品种中的表达差异。结果显示:MeJA处理后,LOX2基因的表达水平在24 h达到最高,是处理前的30.3倍,表明LOX2基因的表达受MeJA诱导;相反,LOX2基因的表达受水杨酸类似物BTH的明显抑制。进一步研究表明:LOX2基因在核盘菌接种后期明显诱导上调表达,接种72 h后在高抗品种D083、中高抗品种ZS9和高感品种84039的表达量分别为接种前的5.0倍2、.7倍和1.7倍。因此,初步推测LOX2基因的高表达在油菜对菌核病抗性中起重要作用,参与了茉莉酸介导的菌核病抗性防御反应。
译  名：
Brassica napus LOX2 Gene Expression Induced by Methyl Jasmonate(MeJA),Benzothiadiazole(BTH),and Sclerotinia sclerotiorum
作  者：
Ren Qiu-hong1,HUANG Jun-Yan2,Mao Han2,TIAN Bao-ming1,LIU Sheng-yi2 (1.Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,China;2.Oil Crops Research Institute of China Agricultural Academy,Chinese Ministry of AgricultureKey Laboratory of Oil Crop Biology,Wuhan 430062,China)
关键词：
Brassica napus;Sclerotinia sclerotiorum;MeJA;BTH;LOX2
摘  要：
Using real-time quantitative PCR,we detected the lipoxygenase2 gene(LOX2) expression levels in Brassica napus after treatment with Methyl jasmonate(MeJA),Benzothiadiazole(BTH) or Sclerotinia sclerotiorum.The results indicated that LOX2 expression was induced by MeJA and suppressed by salicylic acid analogues BTH.After 24 hours of MeJA treatment,LOX2 expression level reached the maximum which was 30.3 fold higher than untreated control.Furthermore,expression levels of LOX2 were detected in three varieties of oilseed rape inoculated with S.sclerotiorum.The results showed that LOX2 was significantly induced after incubation with S.sclerotiorum for 72 hours.With regard to high-resistant variety D083,middle-resistant variety ZS9 and high-susceptible variety 84039,expression levels of LOX2 were 5-fold,2.7-fold and 1.7-fold correspondingly higher than non-incubation plants.These studies suggested that the high expression of LOX2 in oilseed rape may play a key role in defense against S.sclerotioru,and LOX2 may be involved in JA-mediated defense against S.sclerotiorum.

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