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番茄红素的微生物合成及发酵生产研究进展

作  者:
吴军林;吴清平;张菊梅;莫树平;柏建玲
单  位:
广东省微生物研究所;广东省华南应用微生物重点实验室-省部共建国家重点实验室培育基地;广东省微生物菌种保藏与应用重点实验室;广东省微生物新技术公共实验室;广东环凯微生物科技有限公司
关键词:
番茄红素;微生物;合成;基因工程
摘  要:
微生物发酵法是番茄红素生产的最佳方法。番茄红素的生物合成途径有2-甲基-D-赤藓糖醇-4-磷酸盐(MEP)及甲羟戊酸(MVA)两种途径。异戊烯焦磷酸异构酶、牦牛儿基焦磷酸合成酶、法呢基焦磷酸合成酶、牦牛儿基牦牛儿基焦磷酸合成酶、八氢番茄红素合成酶、八氢番茄红素去饱和酶、ζ-胡萝卜素去饱和酶和胡萝卜素异构酶等是番茄红素合成的关键酶。通过诱变、基因重组和基因敲除等方法调节修饰生产番茄红素的关键酶来改变番茄红素合成工艺及其产量。近年来,在利用细菌、霉菌、酵母等微生物发酵生产番茄红素方面进行了大量研究,并研发出了一些新的番茄红素发酵生产工艺,为提高番茄红素产量及降低生产成本提供了良好启发。
译  名:
Advances on Microbial Biosynthesis and Fermentation Production of Lycopene
作  者:
WU Jun-lin;WU Qing-ping;ZHANG Ju-mei;MO Shu-ping;BAI Jian-ling;State Key Laboratory of Applied Microbiology (Ministry-Guangdong Province Jointly Breeding Base)South China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbidogy,Guangdong Institute of Microbiology;Guangdong Huankai Microbial Sci.&Tech. Co. Ltd.;
关键词:
lycopene;;microorganism;;biosynthesis;;gene engineering
摘  要:
Microbial fermentation is the optimal method for lycopene production. There are two biosynthetic pathway of lycopene including MEP(2-methyl-derythritol-4-phosphate) pathway and MVA(Mevalonic acid) pathway. The key relevant enzymes for lycopene biosynthesis includes isopentenyl diphosphate isomerase(IPI), geranyl pyrophosphate sythase(GPS), farnesyl pyrophosphate sythase(FPS), geranylgeranyl pyrophoaphate sythase(GGPS), phytoene synthase(PSY), phytoene desaturase(PDS), ζ-carotenedesaturase(ZDS) and carotene isomerase(CRTISO). The synthesis pathway and the yield of lycopene could be improved by regulating and modifying the key enzymes through mutation, genetic recombination and gene knockout method. In recent years, extensive research has been carried on microbial fermentation production of lycopene using bacteria, fungi, yeast and other microorganisms and several novel technologies for fermentation synthesis of lycopene are developed, which provides insights for improving the yield and reducing the costs of lycopene. This article reviewed the synthesis and production of lycopene by microbial fermentation and the aim was to provide new ideas and directions on development and application of lycopene.

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