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当前位置: 首页 > 文章 > 鸡源高产淀粉酶丁酸梭菌的分离鉴定 中国畜牧兽医 2015,42 (11) 3073-3079
Position: Home > Articles > Isolation and Identification of Producing High-yielding Amylase Clostridium butyricumfrom Chicken China Animal Husbandry & Veterinary Medicine 2015,42 (11) 3073-3079

鸡源高产淀粉酶丁酸梭菌的分离鉴定

作  者：
李圣杰;丁轲;姜芳芳;魏嘉敏;李旺;李元晓;孙二刚
单  位：
河南宏翔生物科技有限公司;河南科技大学宏翔发酵饲料实验室
关键词：
丁酸梭菌;鸡源;淀粉酶;分离;鉴定
摘  要：
为了获得产淀粉酶的丁酸梭菌,本研究从鸡小肠表面黏液中进行丁酸梭菌的分离与筛选,首先对样品进行80℃水浴10min除去非芽孢菌后,然后接种到TSN培养基进行菌株的分离与筛选。对分离到的菌株进行菌落形态、显微形态初步观察,然后对疑似丁酸梭菌的菌株进行产酸能力和淀粉酶酶活检测,最后对既产酸又高产淀粉酶的菌株进行生理生化鉴定和16SrDNA序列分析。结果表明分离到一株革兰氏阳性厌氧菌C.B1,菌体呈短杆状,能形成圆形或椭圆形芽孢,生理生化结果也符合丁酸梭菌的基本特征,16SrDNA序列长度为1 450bp,与丁酸梭菌的同源性高达99%以上,因此确定该分离菌株为丁酸梭菌,为进一步开发新的微生态制剂奠定了基础。
译  名：
Isolation and Identification of Producing High-yielding Amylase Clostridium butyricumfrom Chicken
作  者：
LI Sheng-jie;DING Ke;JIANG Fang-fang;WEI Jia-min;LI Wang;LI Yuan-xiao;SUN Er-gang;Hongxiang Biological Feed Laboratory of Henan University of Science and Technology;Henan Hongxiang Bio-Sci & Tech Co.,Ltd.;Key Laboratory of Animal Disease and Public Health of Henan Province;
关键词：
Clostridium butyricum;;chicken;;amylase;;isolation;;identification
摘  要：
In order to obtain a producing amylase Clostridium butyricum,the chicken small intestinal mucous was collected to isolate and screen Clostridium butyricum.First of all,the samples were heated at 80 ℃for 10 min for removing most non-spore bacteria,then the samples were inoculated on TSN medium.The suspected Clostridium butyricum strains were screened by colony morphology and microscopic morphology observation.The producing acid ability and amylase activity of the isolates were successively tested,so the C.B1 strain,both producing acid and producing high-yielding amylase,was selected to be identified by physiological and biochemical test and 16 SrDNA sequence analysis.The results showed that C.B1 strain had the characteristics of gram-positive anaerobe,rod-shaped and forming a circular or oval spore,16 SrDNA sequence was1 450 bp.Homology comparison analysis indicated that it was most closest to Clostridium butyricum with 99% homology.So C.B1 strain was identified as Clostridium butyricum,this study would lay the foundation for developing new microecological preparations.

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