Effect of Stropharia rugoso-annulata Extract(SRE) on Activities and Isozyme Profiles of Antioxidant Enzymes in Mice with Carbon Tetrachloride-induced Acute Liver Injury

WANG Feng1,WANG Xiao-wei1,TAO Ming-xuan1,ZHANG Chao-ying2,CHENG Guang-yu2(1.Ginling College,Nanjing Normal University,Nanjing 210097,China;2.School of Life Science,Nanjing Normal University,Nanjing 210097,China)

Stropharia rugoso-annulata extract;acute liver injury;antioxidant enzyme;isozyme;gradient polyacrylamide gel electrophoresis

Objective:To evaluate the effect of Stropharia rugoso-annulata extract(SRE) on antioxidative enzyme activity and isozyme profile in liver and other major visceral organs of mice with CCl4-induced acute liver injury.Methods:After 4 days of normal feeding,CIR female mice(25 ± 2) g were used as experimental animals.Ten CIR mice orally administered distilled water were served as control group.Fifty-six more CIR mice were randomly divided into 4 groups of 14 mice each:SRE groups orally administered SRE solution in distilled water at low(100 mg/(kg bw·d)),medium(200 mg/(kg bw·d)) and high(400 mg/(kg bw·d)) doses and model group orally administered distilled water.All mice were administered for 30 consecutive days and low-,medium-and high-dose SRE groups and model group were fed water fasting diet for 16 h on the 30th day before oral administration of CCl4 dispersed in salad oil for creating CCl4-induced acute liver injury model.At 24 h postadministration,all mice were sacrificed to assay activities of antioxidant enzymes in liver,heart,kidney and spleen and analyze isozyme profile of CAT and SOD by gradient polyacrylamide gel electrophoresis.Results:Compared with the model group,high-dose SRE group exhibited significantly increased activities of total SOD,Mn-SOD,CuZn-SOD,GPx and CAT in liver;In addition,there was a significant increase of activities of SOD,CAT and GPx in heart and kidney in three SRE groups.Compared with the normal group,the model group exhibited a compensatory increase of Mn-SOD in kidney;however,Mn-SOD activity was decreased to the normal group level after SRE administration.One CAT band was detected in liver,kidney and heart and CCl4-induced acute liver injury resulted in a significant decrease of staining intensity of the brand.In contrast,the staining intensity of the band was increased to the normal group level after SRE administration.Similarly,one Mn-SOD and three CuZn-SOD bands were detected in liver and the staining intensity of the isozymes was also increased to the normal group level after SRE administration.One Mn-SOD and four CuZn-SOD bands were detected in heart and kidney and SRE administration resulted in the increase of staining intensity of CuZn-SOD bands.Conclusion:SRE can protect liver and other major visceral organs of mice with CCl4-induced acute oxidative injury by enhancing the activities of antioxidant enzymes and the contents of isozymes.