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当前位置: 首页 > 文章 > 陕西省鸡肾型传染性支气管炎病毒的分离鉴定 中国家禽 2007,29 (6) 13-16
Position: Home > Articles > Isolation and Identification of Avian Nephropathogenic Infectious Bronchitis Virus from Shaanxi Province China Poultry 2007,29 (6) 13-16

陕西省鸡肾型传染性支气管炎病毒的分离鉴定

作  者：
罗艳;王晶钰;邓晓敏;姜向阳;张彦明
单  位：
西北农林科技大学动物科技学院
关键词：
肾型传染性支气管炎病毒;分离鉴定;鸡
摘  要：
从临床表现呼吸道传染病的20个发病鸡群中先后分离出18株病毒,通过鸡胚感染、血清学试验、分子生物学试验、动物回归试验证明其中13株是冠状病毒科的鸡传染性支气管炎病毒,3株为新城疫病毒,2株为H9禽流感病毒。该13株中的BJ2、FF2、YL2、B01、G5、YX、WH、WG分离毒具有很强的嗜肾性和致病力,这8个陕西流行株的血清型与W118毒株相同,而与IBVGray株、W93株有一定的差异。结果表明,陕西地区主要流行鸡肾型传染性支气管炎,且流行株具有强致病力。
译  名：
Isolation and Identification of Avian Nephropathogenic Infectious Bronchitis Virus from Shaanxi Province
作  者：
Luo Yan,Wang Jingyu**,Deng Xiaomin,Jiang Xiangyang,Zhang Yanming (College of Animal Science and Veterinary Medicine,Northwest A & F University,Yangling,Shaanxi 712100)
关键词：
nephropathogenetic infectious bronchitis virus;isolation and identification;chicken
摘  要：
Eighteen isolates were isolated successively from 20 suspected flocks with respiratory infection symptoms,and 13 of them were identified to be of infectous bronchitis virus(IBV),which belonged a member of the family Coronaviridae,3 of the rest were Newcastle disease virus(NDV),the last 2 were H9 subtype of avian influenza virus. By chicken embryo infection,serological test,molecular biological test and animal infection tests,the results of animal infection test showed that the 8 isolates,including BJ2,FF2,YL2,B01,G5,YX,WH and WG,had strongly nephropathogencity,moreover,the results of neutralization test in embryonated eggs revealed the control strain W118 could neutralize the 8 isolates completely,while Gray and W93 could not do that,they were able to neutralize W118 to a considerable high degree. In conclusion,the main prevalent strain of IBV in Shaanxi province was nephropathogenetic infectious bronchitis virus.

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