Position: Home > Articles > Mitochondrial DNA polymorphism and genetic relationship of Guizhou sheep
Southwest China Journal of Agricultural Sciences
1999,12
(1)
贵州绵羊线粒体DNA(mtDNA)多态性研究
作 者：
贾永红;简承松;朱文适;张亚平;史宪伟;余应淮;廖正录;李通权
单 位：
贵州省农业厅畜牧局;贵州大学动物科学系;中国科学院昆明动物研究所细胞与分子进化开放研究室
关键词：
贵州绵羊；mtDNA；RELP
摘 要：
采用１５种限制性内切酶对贵州威宁绵羊和六盘水绵羊各５只个体的ｍｔＤ－ＮＡ多态性进行研究。其中只有ＢａｍＨⅠ一种酶的酶切类型表现多态，产生三种酶切类型。共得到１７种限制性态型，可归结为三种ｍｔＤＮＡ单倍型，在威宁绵羊中单倍型Ⅰ所占比较较大（８０％），单倍型Ⅲ只占２０％，而在六盘水绵羊中则单倍型Ⅰ和Ⅱ所占比例相当；根据单倍型在群体中的分布和单倍型间遗传距离，推测贵州绵羊的ｍｔＤＮＡ单倍型Ⅰ和Ⅱ可能代表两个母系祖先的基本单倍型，其分化时间约有１６～３２万年之久，单倍型Ⅲ则可能是由单倍型Ⅰ随机突变而来；两个地方绵羊群体间的分化时间大约为６．３～１２．６万年，群体间的ｍｔＤＮＡ遗传多态度较低，但六盘水绵羊群体内遗传多态度比威宁绵羊较丰富。
译 名：
Mitochondrial DNA polymorphism and genetic relationship of Guizhou sheep
作 者：
JIA Yong hong 1,2) JIAN Cheng song 2) ZHU Wen shi 2) ZHANG Ya ping 1) SHI Xian wei 1) YU Ying huai 2) LIAO Zheng lu 3) LI Tong quan 3) 1) Animal Science Department in Guizhou University,South Di
关键词：
Guizhou sheep;mitochondrial DNA;restriction fragment length polymorphism(RFLP)
摘 要：
In this study 15 restriction enzymes were employed to investigate the mitochondrial DNA polymorphism among the 10 individuals from 2 local Guizhou sheep populations.Only one enzyme (BamH Ⅰ)was found to be polymorphous,which expressed three mtDNA restriction morphs.Seventeen restriction patterns were found that they could be sorted into 3 mtDNA haplotypes.The proportion of haplotype Ⅰ in Weining sheep is higher(80%),but that of haplotype Ⅲ is lower(20%),the proportion of haplotype Ⅰ and Ⅱ are almost equal among Liupanshui sheep.According to the proportion of haplotypes in the population and the genetic distances between the haplotypes,it is deduced that the mtDNA haplotype Ⅰ and Ⅱ are probably representing two mother ancestors of Guizhou sheep,which might separate around 160～320 thousand years ago,haplotype Ⅲ is probably coming from haplotype Ⅰ by random mutation;Two local Guizhou sheep populations separated about 63～126 thousand years ago,and the heterogeneity among the population is low,of which Liupanshui sheep is higher than Weining sheep.
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