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Position: Home > Articles > Staining and discrimination of Nosema bombycis spores with Calcofluor White M2R Acta Entomologica Sinica 2007,50 (11) 1185-1186

Calcofluor White M2R荧光染色法识别家蚕微孢子虫

作  者:
刘吉平;曾玲
单  位:
华南农业大学昆虫生态研究室;华南农业大学动物科学学院
关键词:
家蚕微孢子虫;Calcofluor White M2R;荧光染色;识别技术
摘  要:
本研究探讨应用荧光染色试剂Calcofluor White M2R染色鉴别家蚕微孢子虫Nosema bombycis。结果表明:在荧光显微镜下可见家蚕微孢子虫孢子被染上强烈的青蓝色荧光,而寄主组织碎片、病毒、细菌等不被染色。该法是一种快速有效鉴别微孢子虫的方法。
译  名:
Staining and discrimination of Nosema bombycis spores with Calcofluor White M2R
作  者:
LIU Ji-Ping~ 1,2 , ZENG Ling~2? (1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China; 2. Laboratory of Insect Ecology, South China Agricultural University, Guangzhou 510642, China)
关键词:
Nosema bombycis; Calcofluor White M2R; fluorescent staining; diagnosis
摘  要:
The fluorescent dye Calcofluor White M2R was applied to stain and identify Nosema bombycis spores. The results showed that the spores were stained in green-blue fluorescence. With this method it is easy to distinguish microsporidian spores from virus, bacteria and fungus spores.

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