作 者：
陈海生;陈韬略;蔡林生;李振宇;陈少平;朱友聪;黄霄宇;李晨阳
单 位：
浙江农林大学数学与计算机学院;浙江同济科技职业学院;浙江省余姚第二职业中学;河南职业技术学院
关键词：
鳌江;河岸植被缓冲带;细菌群落结构;细菌多样性;高通量测序
摘 要：
研究了浙江省鳌江流域河岸缓冲带湿地植物根际土壤细菌群落结构及其多样性，以当地河岸缓冲带湿地3种乡土禾本科植物荻草、牛筋草和白芧根际土壤样品中提取的总DNA为模板，运用Illumina Miseq高通量测序技术，对细菌16S rDNA基因V4+V5进行了测序和分析。结果表明，从3种植物根际土壤样品中共测得28门、87纲、168目、296科、696属土壤细菌，其中荻草根际土壤样品的Chao1指数和Shannon指数为最高，其次是白茅根际土壤样品的，牛筋草根际土壤样品的最低。各植物根际土壤细菌群落组成均以变形菌门、酸杆菌门为主，其次为相对丰度较高的放线菌门、厚壁菌门、拟杆菌门、绿弯菌门、芽单胞菌门、硝化螺旋菌门、疣微菌门。从纲水平来看，3种植物根际土壤细菌菌群均以α-变形菌纲，β-变形菌纲及γ-变形菌纲为优势菌纲，其他相对丰度较高的菌纲包括放线菌纲、δ-变形菌纲、芽孢杆菌纲、芽单胞菌纲。3种植物中，变形菌门和硝化螺旋菌门的相对丰度以荻草根际土壤样品的最高，说明荻草在缓冲带湿地脱氮除磷方面可能发挥着更重要的作用，绿弯菌门和酸杆菌门的相对丰度以牛筋草根际土壤样品的最高，说明该植物在缓冲带湿地中能起到更明显的降解河流污染物的作用。
作 者：
CHEN Hai-sheng;CHEN Tao-lue;CAI Lin-sheng;LI Zhen-yu;CHEN Shao-ping;ZHU You-cong;HUANG Xiao-yu;LI Chen-yang;Zhejiang Tongi Vocational college of Science and Technology;School of Mathematics and Computer Science, Zhejiang Agriculture and Forestry University;Yuyao Second Vocational Middle School;Henan Polytechnic;
关键词：
Aojiang River;;Riparian vegetation buffer zone;;Bacterial community structure;;Bacterial diversity;;Highthroughput sequencing
摘 要：
To investigate bacterial community structure and diversity of soil rhizosphere of different plants including Eleusine indica, Miscanthus sacchariflorus and Imperata cylindrica growing in riparian vegetation buffer zones of Aojiang River Basin, Zhejiang Province, the V4+V5 areas of 16S rDNA gene of bacteria were sequenced and analyzed by IlluminaMiseq high-throughput sequencing technology. The results showed that 28 phylums, 87 classes, 168 orders, 296 families and 696genera were obtained from the soil rhizosphere of different plants. The Chao1 index and Shannon index of soil rhizosphere of Miscanthus sacchariflorus were the highest, followed by Imperata cylindrica, and Eleusine indica was the lowest. Proteobacteria and Acidobacteria were the dominant bacterial communities in rhizosphere soil, followed by Actinobacteria, Firmicutes,Bacteroidetes, Chloroflexia, Gemmatimonadetes, Nitrospirae and Verrucomicrobia with relatively high abundance. From the perspective of class level, α-Proteobacteria,β-Proteobacteria, γ-Proteobacteria were the dominant classes in the rhizosphere soil of the three plant species, followed by Actinomycetes, δ-Proteobacteria, Bacillus and Blastomonas with relatively high abundance. The soil rhizosphere of Miscanthus sacchariflorus contained the highest relative abundance of Proteobacteria and Nitrospirae among three plant species, indicating that Miscanthus sacchariflorus plays more important roles in nitrogen and phosphorus removal in riparian wetlands, while the soil rhizosphere of Eleusine indica contained the highest relative abundance of Chloroflexia and Acidobacteria, indicating that Eleusine indica plays more important roles in pollutants degradation of river.
