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当前位置: 首页 > 文章 > 香石竹切花水孔蛋白基因DcPIP2的克隆及特征分析 园艺学报 2011,38 (11) 2199-2208
Position: Home > Articles > Cloning and Characterization of Aquaporin Gene DcPIP2 in Cut Carnations Acta Horticulturae Sinica 2011,38 (11) 2199-2208

香石竹切花水孔蛋白基因DcPIP2的克隆及特征分析

作  者：
李红梅;丁岳炼;黄新敏;林燕飞;邹洁云;何生根
单  位：
仲恺农业工程学院生命科学学院
关键词：
香石竹;切花;水孔蛋白;基因克隆
摘  要：
采用RT-PCR和RACE技术从香石竹(Dianthus caryophyllus L.)叶片中获得质膜内在蛋白(plasma membrane intrinsic protein,PIP)类水孔蛋白(aquaporins,AQP)基因的cDNA全长序列,命名为DcPIP2,GenBank登录号为GU989036。该cDNA全序列长983bp,包含有858bp的完整阅读框(ORF),编码285个氨基酸,分子量约为30.6kD。克隆相应的DcPIP2基因组全长序列得知,该基因长2635bp(GenBank登录号为JF706350),包含由3个外显子和2个内含子组成的编码区序列。同源性分析显示,DcPIP2氨基酸序列与陆地棉(Gossypium hirsutum)PIP2;3(ACB42440)、麻疯树(Jatropha curcas)AQP(ABM54183)和巴西橡胶树(Hevea brasiliensis)PIP2(ACV66986)氨基酸的同源性分别为89%、88%和87%。利用实时荧光定量PCR技术研究表明,DcPIP2基因在香石竹切花的叶片、茎颈部、花瓣、雌蕊、雄蕊和萼片中均有表达,表达量为雌蕊>花瓣>雄蕊>萼片>茎颈部>叶片。
译  名：
Cloning and Characterization of Aquaporin Gene DcPIP2 in Cut Carnations
作  者：
LI Hong-mei,DING Yue-lian,HUANG Xin-min,LIN Yan-fei,ZOU Jie-yun,and HE Sheng-gen (College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)
关键词：
Dianthus caryphyllus L.;carnation;cut flower;aquaporin;gene cloning
摘  要：
A PIP(plasma membrane intrinsic protein)aquaporins(AQP)gene,designated as DcPIP2 (GenBank accession number GU989036),was cloned from carnation(Dianthus caryophyllus‘Master’) leaves by RT-PCR and RACE. The full cDNA sequence of DcPIP2 is 983 bp,containing an open reading frame(858 bp)and encoding a protein of 285 amino acids with a predicted molecular mass of 30.6 kD. The DcPIP2 genomic DNA(2 635 bp)(GenBank accession number JF706350),corresponding to the DcPIP2 cDNA,was further cloned,which contains three exons and two introns in its coding sequence. The sequence analysis showed that the homology of amino acid sequences between DcPIP2 and Gossypium hirsutum PIP2;3(ACB42440),Jatropha curcas AQP(ABM54183),and Hevea brasiliensis PIP2(ACV66986)was 89%,88% and 87%,respectively. The expression of DcPIP2 in different organs of cut carnation flowers was determined by real-time quantitative PCR,it was found that DcPIP2 gene wasexpressed in leaves,stem necks,petals,pistils,stamina and sepals. The highest expression level of DcPIP2 was detected in pistils,followed by petals,while moderate expression level was present in stamina and stem necks,and the lowest level was in leaves.

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