Xu DanDan;Bo Zhang;Huang YaDong;Qi-Hao Zhang;Zhijian Su;Xu Hua;Zheng Qin

curcin;recombinant;recombinant protein;expressed;jatropha curcas l.;expressio

[Objective] To study the expression of recombinant curcin in Jatropha curcas L.and its antitumour activity in vitro.[Method] Curcin was cloned from the leaves of J.curcas,and the recombinant vector pET-22b was established.The recombinant protein(29KD) was expressed in E.coli.[Result] Induced by 0.5 mM IPTG for 6 h at 30 ℃,expressing quantity of recombinant protein was about 20% of the total protein.Recombinant curcin was expressed in the form of inclusion body.After dissolution,renaturation and purification,purity of recombinant curcin reached 95.6%.[Conclusion] In vitro research showed that recombinant protein had significant proliferation inhibition effects on the A549 and HepG2 tumor cells.And their IC50 were 92.246 and 691.42 μg/ml,respectively.But the recombinant protein had no significant inhibitory effects on Hela.