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当前位置: 首页 > 文章 > 4型禽腺病毒抗体间接hexon-ELISA检测方法的建立 中国兽药杂志 2018 (8)
Position: Home > Articles > 4型禽腺病毒抗体间接hexon-ELISA检测方法的建立 Chinese Journal of Veterinary Drug 2018 (8)

4型禽腺病毒抗体间接hexon-ELISA检测方法的建立

作  者：
朱庆贺;张鹏宇;崔红玉;王观悦;王爽;陈曦;刘力威;史同瑞
关键词：
4型禽腺病毒;hexon蛋白;原核表达;间接ELISA
摘  要：
以4型禽腺病毒(FAdV-4)的DNA为模板,扩增其六邻体(hexon)部分基因并进行重组表达,以重组hexon蛋白为包被抗原,优化ELISA检测条件,建立了FAdV-4的ELISA抗体检测方法.该方法对FAdV-4阳性血清检测为阳性,对于其他鸡常见病毒,如禽流感病毒5、7、9型,新城疫病毒以及鸡传染性支气管炎病毒阳性血清检测均为阴性;与PCR方法的阳性符合率为83.3%.试验表明,建立的以重组hexon蛋白为包被抗原检测FAdV-4血清抗体的ELISA方法可以用于检测FAdV-4的感染及相关流行病学调查.
作  者：
ZHU Qing-he;ZHANG Peng-yu;CUI Hong-yu;WANG Guan-yue;WANG Shuang;CHEN Xi;LIU Li-wei;SHI Tong-rui;Heilongjiang Institute of Veterinary Science;Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences;
单  位：
ZHU Qing-he%ZHANG Peng-yu%CUI Hong-yu%WANG Guan-yue%WANG Shuang%CHEN Xi%LIU Li-wei%SHI Tong-rui%Heilongjiang Institute of Veterinary Science%Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences
关键词：
FADV-4;;hexon protein;;prokaryotic;;indirect ELISA
摘  要：
FAdV-4 DNA as templates was used to amplify the hexon gene,and the recombinant hexon protein was expressed.With the recombinant hexon protein as the detection antigen,an indirect ELISA was established by optimizing the ELISA detection conditions.FAdV-4 antibodies reacted with the hexon protein significantly,no cross-reactivity occurred between the hexon protein and antibodies against other chicken viruses,such as avian influenza virus(AIV-5,AIV-7,AIV-9),newcastle disease virus(NDV),and infection bronchitis virus(IBV).The positive coincidence rate with PCR method was 83.3%.It showed that the developed ELISA could be used for the diagnosis of FAdV-4 infection and investigation of related epidemiological.

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