中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 水稻白叶枯病原菌遗传和致病型多样性分析 华南农业大学学报 2005,26 (2) 26-30
Position: Home > Articles > Analysis of genotypic and pathotypic diversity in Xanthomonas oryzae pv.oryzae by Rep-PCR Journal of South China Agricultural University 2005,26 (2) 26-30

水稻白叶枯病原菌遗传和致病型多样性分析

作  者：
姬广海;何月秋;张世光;钱君
单  位：
云南农业大学云南省植物病理重点实验室
关键词：
水稻白叶枯病;遗传多样性;致病型;Rep-PCR
摘  要：
采用Rep PCR分子指纹技术对国内已知致病型和云南省各水稻产区采集的5 6个白叶枯病代表菌株进行分析.用ERIC、J3等引物对其DNA进行Rep PCR特异性扩增,扩增结果经综合聚类分析,在70 %的相似水平上,可将测试菌株划分为10个组群,其中4、5、8、9、10为主要组群;来自云南省的菌株分属于6个组群中,遗传分群与菌株的地理来源关系密切.根据菌株在已知抗性基因的近等基因系的反应,菌株可分为18个致病型,云南省的水稻白叶枯病菌株致病型复杂多样,远远高于国内其他省份测试的致病型
译  名：
Analysis of genotypic and pathotypic diversity in Xanthomonas oryzae pv.oryzae by Rep-PCR
作  者：
JI Guang-hai, HE Yue-qiu, ZHANG Shi-guang, QIAN Jun(Key Lab for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China)
关键词：
Xanthomonas oryzae pv. oryzae; genetic diversity; pathotype; Rep-PCR
摘  要：
Fifty-six strains of Xanthomonas oryzae pv. oryzae (Xoo) were analysed by using short bacterial repetitive elements(REP,ERIC and BOX primers) and the repetitive DNA element IS1113 from X. oryzae pv.oryzae. Dendrograms were generated from the combination data sets of primers ERIC and J3 by using UPGMA analysis. The results indicated that extensive genetic variability and genetic diversity existed within the population of X. oryzae pv. oryzae strains tested. All strains could be divided into 10 groups at level of 70% similarity. Group 4, 5, 8, 9 and 10 were predominant. Strains collected from Yunnan Province were divided into 6 groups, and each group was partially associated with the regional origin of pathogen population. Eighteen pathotypes (virulence phenotype) were identified bg using 16 near-iso-genic rice lines, each containing a single gene for resistance. The pathotype diversity and complicity of strains tested from Yunnan Province were higher than those from other provinces in China. The results would be facilitated for further understanding of the population structure of X.oryzae pv.oryzae in China.

相似文章

导出引用
计量
文章访问数: 4
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46