中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 牛源大肠杆菌质粒介导喹诺酮类耐药基因的检测分析 中国畜牧兽医 2019 (8) 2462-2469
Position: Home > Articles > 牛源大肠杆菌质粒介导喹诺酮类耐药基因的检测分析 China Animal Husbandry & Veterinary Medicine 2019 (8) 2462-2469

牛源大肠杆菌质粒介导喹诺酮类耐药基因的检测分析

作  者：
杨艳丽;张星星;黄新;吴桐忠;何延华;韩猛立;钟发刚
单  位：
新疆农垦科学院省部共建绵羊遗传改良与健康养殖国家重点实验室
关键词：
新疆;牛源大肠杆菌;喹诺酮类药物耐药基因;药敏试验
摘  要：
为研究近年来新疆地区牛源大肠杆菌中质粒介导喹诺酮类药物耐药基因的分布及其对喹诺酮类抗生素的耐药情况,本研究于2016-2018年从新疆石河子、沙湾、奎屯、玛纳斯和伊犁5个地区12个规模化奶牛场分离出116株牛源大肠杆菌,药敏试验检测其耐药性,同时利用PCR扩增PMQR耐药基因.药敏试验结果显示,62.93%的菌株对氨苄西林耐药,耐药率最高.对链霉素、四环素、卡那霉素和恩诺沙星的耐药率依次为56.90%、54.31%、43.10%和42.24%.对头孢他啶和头孢噻肟的耐药率较低,分别为7.76%和11.21%.分离菌主要携带qnrA、qnrS和aac(6′)-Ⅰb-cr 3种耐药基因;116株大肠杆菌中有31株携带PMQR的耐药基因,检出阳性率为26.72%,其中26株仅携带1种PMQR耐药基因,占所有菌株的22.41%,4株携带2种PMQR耐药基因,占所有菌株的3.45%,1株携带3种PMQR耐药基因,占所有菌株的0.86%.综上所述,新疆地区牛源大肠杆菌质粒介导喹诺酮类药物基因主要为qnrA、qnrS和aac(6′)-Ⅰb-cr 3种,且对恩诺沙星、诺氟沙星、环丙沙星、左氧氟沙星均产生不同程度的耐药性.
作  者：
YANG Yanli;ZHANG Xingxing;HUANG Xin;WU Tongzhong;HE Yanhua;HAN Mengli;ZHONG Fagang;State Key Laboratory for Sheep Genetic Improvement and Healthy Production,Xinjiang Academy of Agricultural and Reclamation Science;College of Veterinary Medicine,Xinjiang Agricultural University;
关键词：
Xinjiang;;bovine Escherichia coli;;PMQR resistance genes;;drug susceptibility test
摘  要：
In order to understand the gene distribution of plasmid-mediated quinolone resistance and its resistance to quinolone antibiotics in bovine E.coli in Xinjiang in recent years.116 strains of E.coli were isolated from 12 large-scale dairy farms in Shihezi,Shawan,Kuitun,Manas and Yili of Xinjiang in 2016-2018.The PMQR resistance gene was amplified by PCR and its resistance was detected by drug sensitivity test.The results showed that 62.93% of the 116 bovine derived E.coli strains were resistant to ampicillin,which was the strongest among the 15 tested antibiotics followed by streptomycin,tetracycline,kanamycin and enrofloxacin,whose resistance rates were 56.90%,54.31%,43.10% and 42.24%,respectively.The resistance rates to ceftazidime and cefotaxime were lower(7.76% and 11.21%).The PMQR resistance genes carried by the E.coli in Xinjiang were mainly qnrA,qnrS and aac(6′)-Ⅰb-cr.The PCR results showed that there were 31 strains carrying PMQR resistance genes in 116 strains,and the positive rate was 26.72%.26,4 and 1 strains carried 1,2 and 3 kinds of PMQR resistance gene,accounting for 22.41%,3.45% and 0.86% of all strains,respectively.In conclusion,the PMQR resistance genes mediated by the E.coli in Xinjiang were mainly qnrA,qnrS and aac(6′)-Ⅰb-cr,and it was common for the E.coli resistance to enrofloxacin,norfloxacin,ciprofloxacin and levofloxacin in this area.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46